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Simultaneous determination of two active components of pharmaceutical preparations by sequential injection method using heteropoly complexes

Mohammed Khair E. A. Al-Shwaiyat, Tatyana A. Denisenko, Sergey V. Zaruba, Andriy B. Vishnikin, Ludmila P. Tsiganok, Vasil Andruch, Yaroslav R. Bazel


New approach has been proposed for the simultaneous determination of two reducing agents based on the dependence of their reaction rate with 18-molybdo-2-phosphate heteropoly complex on pH. The method was automated using the manifold typical for the sequential analysis method. Ascorbic acid and rutin were determined by successive injection of two samples acidified to different pH. The linear range for rutin determination was 0.6-20 mg/L and the detection limit was 0.2 mg/L (l = 1 cm). The determination of rutin was possible in the presence of up to a 20-fold excess of ascorbic acid. The method was successfully applied to the determination of ascorbic acid and rutin in ascorutin tablets. The applicability of the proposed method for the determination of total polyphenol content in natural plant samples was shown.


spectrophotometric determination; rutin; ascorbic acid; 18-molybdo-2-phosphate heteropoly anion

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