Simultaneous determination of two active components of pharmaceutical preparations by sequential injection method using heteropoly complexes

Authors

  • Mohammed Khair E. A. Al-Shwaiyat Zarka University college, Al-Balqa’ Apllied University, Zarka Amman Highway, Zarka 13110, Jordan
  • Tatyana A. Denisenko Oles Honchar Dnipropetrovsk National University, 72, Gagarin Ave., Dnipropetrovsk 49010, Ukraine
  • Sergey V. Zaruba Oles Honchar Dnipropetrovsk National University, 72, Gagarin Ave., Dnipropetrovsk 49010, Ukraine
  • Andriy B. Vishnikin Oles Honchar Dnipropetrovsk National University, 72, Gagarin Ave., Dnipropetrovsk 49010, Ukraine
  • Ludmila P. Tsiganok Oles Honchar Dnipropetrovsk National University, 72, Gagarin Ave., Dnipropetrovsk 49010, Ukraine
  • Vasil Andruch Pavol Jozef Šafarik University, SK-01454 Košice, Slovakia
  • Yaroslav R. Bazel Pavol Jozef Šafarik University, SK-01454 Košice, Slovakia

DOI:

https://doi.org/10.15421/081408

Keywords:

spectrophotometric determination, rutin, ascorbic acid, 18-molybdo-2-phosphate heteropoly anion

Abstract

New approach has been proposed for the simultaneous determination of two reducing agents based on the dependence of their reaction rate with 18-molybdo-2-phosphate heteropoly complex on pH. The method was automated using the manifold typical for the sequential analysis method. Ascorbic acid and rutin were determined by successive injection of two samples acidified to different pH. The linear range for rutin determination was 0.6-20 mg/L and the detection limit was 0.2 mg/L (l = 1 cm). The determination of rutin was possible in the presence of up to a 20-fold excess of ascorbic acid. The method was successfully applied to the determination of ascorbic acid and rutin in ascorutin tablets. The applicability of the proposed method for the determination of total polyphenol content in natural plant samples was shown.

Author Biography

Sergey V. Zaruba, Oles Honchar Dnipropetrovsk National University, 72, Gagarin Ave., Dnipropetrovsk 49010

M.S. in Chemistry, PhD Student of Analytic Chemistry Department

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Published

2014-12-02

Issue

Vol. 22 No. 1 (2014): Bulletin of Dnipropetrovsk University. Series Chemistry

Section

Analytical Chemistry

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Copyright (c) 2014 Oles Honchar Dnipropetrovsk National University

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