Digital colorimetric determination of vitamin C using 18-molybdo-2-phosphate

Galyna O. Petrushyna, Andriy B. Vishnikin, Ludmyla I. Puhach, Svitlana O. Khmelovs’ka, Darya V. Pidgorna


Reduction with ascorbic acid of tetraethylammonium salt of 18-molybdo-2-phosphate heteropoly complex impregnated on the filter or the chromatographic paper was investigated. Digital scan images of colored paper test strips obtained with the office scanner were resolved into R, G, and B colors. It was found that dependence of the coordinates of RGB colors on the ascorbic acid concentration is satisfactorily linearized using the equation y = yo + Aexp(–c/t). Rapid and simple colorimetric method for the determination of ascorbic acid based on its reaction with impregnated on the paper 18-MPC was developed. Method was applied to the determination of vitamin C in juices.


18-molybdo-2-phosphate; heteropoly blue; chromaticity; vitamin C; ascorbic acid


Zolotov, Yu. A., Ivanov, V. M., & Amelin, V. G. (2002). [Chemical Test Methods of Analysis]. Moskow, Russian Federation: Editorial URSS (in Russian).

Zaporozhets, О. А., & Krushinskaya, Е. А. (2002). Determination of Ascorbic Acid by Molecular Spectroscopic Techniques. J. Anal. Chem., 57(4), 286–297. doi:10.1023/A:1014938011955 CrossRef

Arya, S. P., Mahajan, M., & Jain, P. (1998). Photometric Methods for the Determination of Vitamin C. Anal. Sci., 14(5), 889–895. doi:10.2116/analsci.14.889 CrossRef

Ikeda, Y. O., & Yao, K. I. (1981). US Patent No. 140675. Osaka, Japan: Shionogi & Co. Ltd.

Ordman, A. B., & Weiss, H. M. (1998). US Patent No. 709257. Scottsdale, Ariz.: Weiss, H. M.

Deneke, U., Michal, G., & Beauchamp, K. (1979). US Patent No. 920034. Mannheim-Waldhof, Fed. Rep. of Germany: Boehringer Mannheim GmbH.

Vishnіkіn, A. B., & Svinarenko, T. Ye. (2008). Ukraine Patent No. 37588. Dnipropetrovsk, Ukraine. Dnipropetrovsk National University.

Petrushina, G. A., Tsiganok, L. P., & Vishnikin, A. B. (2010). [Spectrophotometric determination of ascorbic acid by using P2Mo18O626–]. Ukr. Chem. J., 76(4), 116–122 (in Russian).

Vishnikin, A. B., Sklenařova, H., Solich, P., Petrushina, G. A., & Tsiganok, L. P. (2011). Determination of ascorbic acid with Wells-Dawson type molybdophosphate in sequential injection system. Anal. Lett., 44(1–3), 514–527. doi:10.1080/00032719.2010.500789 CrossRef

Chebotaryov, A. N., Snigur, D. V., Bevziuk, E. V., & Efimova, I. S. (2014). [The Trends Analysis of Chemical Chromaticity Method Evolution (Review)]. Metody Ob'ekty Khim. Anal. – Methods Objects Chem. Anal., 9(1), 4–11 (in Russian).

Rudakova, L. V., & Rudakov, O. B. (2015). [Determination of paracetamol and sodium salicylate using TLC, photometry and digital colorimetry]. Vestn. Voronezh. Gos. Univ.: Khim. Biol. Farm. – Proceedings of Voronezh State Univ.: Chem. Biol. & Pharm., (2), 35–41 (in Russian). Retrieved from

Gorda, R., Trokhymchuk, A., & Legenchuk, O. (2014). [Colorimetric Determination of Pd(II), Rh(III), Ru(IV) on the Surface of Silica Gel Chemically Modified with Thiourea Groups]. Visn. Kyjiv Nats. Univ. im. T. Shevchenka: Khim. – T. Shevchenko Nat. Univ. of Kyiv Chem. Bull., 50(1), 41–43 (in Ukrainian). Retrieved from

Reshetnyak, E. A., Shevchenko, V. M., Pastushkina, I. О., Zagrzewski, P. M., Bondarenko, Ya. A., Vlasova, I. V., & Ostrovskaya, V. M. (2013). [Separate sorption-spectroscopic determination of Pb(II), Mn(II), Co(II), Ni(II), Cd(II), Cu(II), Zn(II) in their mixture using the reagent indicator paper]. Visn. Khark. Nats. Univ. im. V. N. Karazina. Khim. – V. N. Karazin Kharkiv Nat. Univ. Bull.: Chem., 22(1985), 210–219 (in Russian). Retrieved from

Khudyakova, S. N., Kolesnikova, Е. L., & Kholodelshchikova, J. A. (2016). Visual Test and Colorimetric Determination of Gold(III) with the Use of Indicator Paper. Bulletin Dnipropetrovsk Univ. Ser. Chem., 24(1), 36–47 (in Russian). doi:10.15421/081606 CrossRef

Khudyakova, S. N., & Chmilenko, F. A. (2015). Indicator Powder and Indicator Test-Tube on the Basis of Methylsilicic Acid Immobilized with Dimercaptothiopyrone Derivatives as Test System for the Colorimetric and Visual-Test Determination of Palladium(II). Vìsn. Dnìpropetr. unìv., Ser. Hìm., 23(1), 30–39 (in Ukrainian). doi:10.15421/081505 CrossRef

Sadakane, M., & Steckhan, E. (1998). Electrochemical Properties of Polyoxometalates as Electrocatalysts. Chem. Rev., 98(1), 219–238. doi:10.1021/cr960403a CrossRef

Petrushina, G. A., Tsiganok, L. P, & Vishnikin, A. B. (2011). [Spectrophotometric determination of p-aminophenol in the presence of paracetamol by using 18-molybdodiphosphate]. Bull. Dnipropetrovsk Univ. Ser. Chem., 19(3/1), 160–164

(in Russian). Retrieved from

Petrushyna, G. O., & Tsiganok, L. P. (2010). Ukraine Patent No. 55028. Dnipropetrovsk, Ukraine. Oles Honchar Dnipropetrovsk National University.

Dmitrienko, S. G., Apyari, V. V., Sviridova, O. A., Badakova, S. A., & Zolotov, Yu. A. (2004). [On the new Approach to the Theory of Preferential Wetting of Heteroheneous Solid Surfaces]. Moscow Univ. Chem. Bull., 45(2), 131–138 (in Russian). Retrieved from

Selivanova, T. V., Vishnikin, A. B., & Tsiganok, L. P. (2009). [Ionic Associates SiMo12O404- with Triphenylmethane Dyes in the Sorption-Colorimetric and Visual Test-Determination of Silicium]. Metody Ob'ekty Khim. Anal. – Methods Objects Chem. Anal., 4(1), 18–22 (in Russian).

Dejaegher, B., Bloomfield, M. S., Smeyers-Verbeke, J., & Vander Heyden, Y. (2008). Validation of a fluorimetric assay for 4-aminophenol in paracetamol formulations. Talanta, 75(1–2), 258–265. doi:10.1016/j.talanta.2007.11.029 CrossRef

Kristian, G. (2009). [Analitycal Chemistry (Vol. 1)]. Мoscow, Russian Federation: Binom (in Russian).

European Pharmacopeia (6th ed.) (2007). European Directorate for the Quality of Medicines (EDQM). Council of Europe, Strasbourg Cedex, France.



  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

eISSN: 2313-4984 | pISSN: 2306-871X
The journal publishes research papers on terms: Creative Commons Attribution 4.0 International License
Founder: Oles Honchar Dnipropetrovsk National University