• Andriy B. Vishnikin Oles Honchar Dnipro National University, Ukraine
  • Larisa P. Sidorova Дніпровський національний університет імені Олеся Гончара, Ukraine
  • Alina O. Voloboy Дніпровський національний університет імені Олеся Гончара, Ukraine



simultaneous determination, synthetic food dyes, E110 (yellow "Sunset"), E124 (Ponso 4R, bright red 4R), spectrophotometry, H-point standard addition method, soft drinks.


A simple, rapid and highly sensitive method for the determination of the content of synthetic food dyes in binary mixtures using the standard H-point additives has been developed. The theoretical foundations of the method are given. The following analytical wavelengths were selected to determine the yellow dye «Sunset» (E 110) and Ponceau 4R (E124) in mixtures: 446 and 521 nm for the dye E110 and 470 and 542 nm for the dye E124. In accordance with the requirements of the method, for these wavelengths, both absorbance and concentration were the same. At the same time, for the other component, the difference in absorbances at the same wavelengths was large enough and the concentration changed systematically. Simultaneous determination of dyes E124 and E110 is possible with satisfactory precision in the range of their concentrations in a mixture from1 to 30 μg/mL. The method is characterized by satisfactory precision, and the relative error does not exceed 4 % when using the SF-46 spectrophotometer. The technique has been successfully tested on artificial mixtures and commercially available carbonated beverage samples: «Mirinda», «Shake», «InStyle», «Fruits Multivitamin».

Author Biographies

Andriy B. Vishnikin, Oles Honchar Dnipro National University

Head of Analytical Chemistry Department h-index (SCOPUS) = 8

Larisa P. Sidorova, Дніпровський національний університет імені Олеся Гончара

Доцент кафедри аналітичної хімії h-index (SCOPUS) = 2

Alina O. Voloboy, Дніпровський національний університет імені Олеся Гончара

Студент хімічного факультету ДНУ


Sanitary norms and rules for the use of food additives. No. 222 of July 23, 1996.

Smirnov, E.V. (2009). Food dyes. Directory. St. Petersburg: Profession (in Russian).

Korenman, Ya. I., Sannikova, N. Yu., Sukhanov, P. T. (2010). [Extraction preconcentration and identification of synthetic dyes in food products by TLC]. Zavodskaya laboratoriya. (6), 16–18 (in Russian).

Kolesnik, A. V., Korenman, Ya. I., Sannikova, N. Yu., Sukhanova, P. T. (2010). Extraction-chromatographic determination of sulfonatedazo dyes in aqueous solutions. J. Anal. Chem. 65(5), 460–465.

Chmylenko, F.O., Minayeva, N.P., Sandomyrsʹkyy, O.V., Sydorova, L.P. (2008). [Identification of dyes in beverages by high-performance liquid chromatography]. Kharchova promyslovistʹ, (7), 17–19 (in Ukrainian).

Minaeva, N.P., Sandomirskii, A.V., Sidorova, L.P., Chmilenko, F.A. (2009). Speedy technique of chromatographic determination of toxic metals in drinking water. J. Water Chem. Technol. 31(5), 305–309.

Kiseleva, M.G., Pimenova, V.V., Eller, K.I. (2003). Optimization of conditions for the HPLC determination of synthetic dyes in food. J. Anal. Chem.58(7), 685–690.

Chmilenko, F.A., Sidorova, L.P., Minaeva, Y.A., Shkurovskaya, K.V. (2014). [Extraction-chromatographic determination of the content of synthetic dyes in food]. Issues of Chemistry and Chemical Technology, (2), 45–49 (in Ukrainian).

Shestopalova, N. B., Petrovich, M. V., Chernova, R. K. (2016). [Determination of synthetic food dyes E102 and E110 in the presence of each other]. Zhurnal Izvestiya Saratovskogo universiteta. Novaya seriya. Seriya Khimiya. Biologiya. Ekologiya, 16(3), 247–252 (in Russian).

Al-Sabha, T.N., Bunaciu, A.A., Aboul-Enein, H. Y. (2011). H-Point standard addition method (HPSAM) in simultaneous spectrophotometric determination of binary mixtures: An overview. Appl. Spectr. Rev., (46), 607–623.

Wieczorek, M., Rengevicova, S., Świt, P., Woźniakiewicz, A., Kozak, J., Kościelniak, Р. (2017). New approach to H-point standard addition method for detection and elimination of unspecific interferences in samples with unknown matrix. Talanta, (170) 165–172.

Oveisi, M. R., Sadeghi, N., Jannat, B., Nilfroush,E. (2008). Simultaneous determination of Carmoisine and Ponceau 4R. Food Anal. Methods.(1), 214–219.

Kaur, A., Gupta, U. (2011). H-Point standard addition method for simultaneous determination of Eosin and Erytrosine. E-Journal of Chemistry, 8(4), 1979–1985.

Kaur, A., Gupta, U. (2012). Determination of binary mixtures of Tartrazine and Quinoline Yellow food colorants in various food samples and cosmetic products in micellar media by H-Point standard addition method (HPSAM). Int. J. Res. Chem. Environ., 2(1), 293–300.

Al-Shwaiyat,M. K. E. A., Miekh, Yu.V., Denisenko, T.A., Vishnikin, A.B., Andruch, V., Bazel, Ya.R. (2018). Simultaneous determination of rutin and ascorbic acid in a sequential injection lab-at-valve system. J. Pharm. Biomed. Anal. 149. 179–184.

Ni, Y., Qi, M., Kokot, S. (2001). Simultaneous spectrophotometric determination of ternary mixtures of tartrazine, sunset yellow, and Ponceau 4R by H-point standard addition method. Anal. Lett., 34(14), 2585–2596.

Vishnikin, А., Miekh, Yu., Denisenko, T., Bazel, Ya., Andruch, V. (2018). Using of sequential injection analysis with lab-at-valve and optical probe for simultaneous spectrophotometric determination of ascorbic acid and cysteine by mean centering of ratio kinetic profiles. Talanta, 188,99–106.

Al-Shwaiyat,M. E. A., Miekh,Yu. V., Denisenko,T. A., Vishnikin,A. B., Bazel,Ya. R. (2017). Simultaneous kinetic determination of ascorbic acid and analgine in pharmaceutical preparations by H-point standard addition method. Bulletin of Dnipropetrovsk university. Series Chemistry, 25(2), 93–102.

Bosch-Reig, F., Campíns Falcó, P. (1988). H-point standard additions method. Fundamentals and application to analytical spectroscopy.Analyst, 113(7), 1011–1016.

Bosch-Reig, F., Campins-Falco, P., Sevillano-Cabeza A., Herraez-Hernandez, R., Molins-Legua, C. (1991). Development of the H-point standard-additions method for ultraviolet-visible spectroscopic kinetic analysis of two-component systems. Anal. Chem., 63(21), 2424–2429.

Khal'zova S. A. (2017). [Determination of synthetic dyes in liquid media by piezoelectric sensors, modified polymers with molecular prints] (Unpublished Ph.D. dissertation). Voronezh state university, Voronezh, Russia (in Russian).

Bevzyuk K. V. (2019) [Solution state, sorption extraction and voltammetric determination of synthetic food colors]. (Unpublished Ph.D. dissertation). Uzhhorod national university, Uzhhorod, Ukraine (in Ukrainian).