DOI: https://doi.org/10.15421/082011

PROBLEMS OF THE DAIRY AND MEAT INDUSTRY MARKET. MONITORING THE SPREAD OF ANTIBIOTIC-RESISTANT STRAINS OF SANITARY-INDICATIVE MICROFLORA

Tetiana V. Sklyar, Kateryna V. Lavrentieva, Olena A. Lykholat, Natalya V. Kondratjuk, Kateryna Ye. Suprunenko, Tetiana М. Stepanova

Abstract


This article presents a study of monitoring the spread of antibiotic-resistant strains of sanitary-indicative microflora of food products sold on the consumer market of Dnipropetrovsk region.

The increase of population in the world leads to an increase in the quality of livestock and poultry products. World experience shows that it is impossible to abandon the use of antibiotics in the rearing of pigs, cattle and poultry. Firstly, it is related to therapeutic and preventive measures in the fight against various diseases. Secondly, due to biochemical transformations in muscle fibers with the participation of antibiotics, consumer quality indicators (color, consistency) and technological quality indicators (moisture-absorbing and moisture-retaining ability) are improved. Therefore, livestock products can be one of the sources of sanitary microflora entering into the human body. In addition to the ability to cause an infectious process due to the synthesis of various pathogenic factors, including enzymes, toxins, adhesion substances, etc., in the sanitary-indicative microflora there is interstitial, intraspecific and intergeneric transfer of antibiotic resistance genes. This contributes to the emergence of cultures with acquired resistance to antibiotics and the formation of multi-resistant variants. Elimination of such strains from the human body will present certain difficulties.

It was found that in the studied samples of milk and dairy products, as well as meat and offal of cattle and poultry, representatives of coliform bacteria, staphylococci and enterococci were less common. The isolated strains of sanitary-indicative microflora were the most resistant to ampicillin, oxacillin, tetracycline and doxycillin. 10 strains of S. aureus and 7 strains of  S. epidermidis was classified as MRSA and MRSE, respectively. Five of them were resistant to vancomycin and three to linezolid. Among the isolated cultures of enterococci were strains that were resistant to 5-6 or more drugs simultaneously. Of the bacteria of the family Enterobacteriaceae, the ability to synthesize extended-spectrum beta-lactamase (ESBL) was detected in 18 cultures, which accounted for 25.0% of the total number of opportunistic enterobacteria. The ESBL strains of bacteria of the family Enterobacteriaceae differed from other isolates by resistance to at least 4 antibiotics, among which at least one belonged to the group of third-generation cephalosporins. 7 strains of Escherichia coli, two strains of P. vulgaris and three strains of K. pneumoniae were characterized by resistance to 6 or more tested drugs.

Keywords


meat products; dairy products; antibiotic resistance; bacterium of intestinal bacillus; Staphylococcus aureus; Staphylococcus epidermidis; Enterobacter cloaceae; Klebsiella pneumoniae; Proteus vulgaris.

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References


Chen, X., Zhu, W., Liu, X., Li, T. (2019). The growth gerformance, meat quality, and gut bacteria of broilers raised with or without antibiotics and green tea powder. Journal of Applied Poultry Research, 28(3), 712–721. http://doi.org/10.3382/japr/pfz023

Bartkiene, E., Ruzauskas, M., Bartkevics, V., Pugajeva, I. (2020). Study of the antibiotic residues in poultry meat in some of the EU countries and selection of the best compositions of lactic acid bacteria and essential oils against Salmonella enterica. Poultry Science, 1–12 http://doi.org/10.1016/j.psj.2020.05.002

Bartkiene, E., Ruzauskas, M., Bartkevics, V., Pugajeva, I. (2020). Study of the antibiotic residues in poultry meat in some of the EU countries and selection of the best compositions of lactic acid bacteria and essential oils against Salmonella enterica. Poultry Science, 1-12 http://doi.org/10.1016/j.psj.2020.05.002

Wang, H., Ren, L., Yu, X., Hu, J. (2017). Antibiotic residues in meat, milk and aquatic products in Shanghai and human exposure assessment. Food Control. 80, 217–225. http://doi.org/10.1016/j.foodcont.2017.04.034

Normanno, G. (2007). Occurrence, characterization and antimicrobial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. International Journal of Food Microbiology. 115(3), 290–296.

https://doi.org/10.1016/j.ijfoodmicro.2006.10.049

Goriuk Yu. V. (2016). [Staphylococcus aureus biotypes isolated from raw milk and home-made dairy products and their sensitivity to antibacterial drugs]. Problemy zooinzhenerii ta veterynarnoi medytsyny – The problems of zooengineering and veterinary medicine, 32(2), 185–190 (in Ukrainian).

Nawras, N. J. (2011). Isolation and biotyping of Staphylococcus aureus from white cheese in basrah local markets. Bas. J. Vet. Res. 88(10), 56–65. https://doi.org/10.33762/bvetr.2011.55025

Kucheruk, M. D., Zasekyn, D. A., Vyhovska, L. M., Ushkalov, V. O. (2018). [Antibiotic resistance of field strains of microorganisms]. Bioresursy i pryrodokorystuvannia. – Bioresources and nature management, 10(5-6), 205–217 (in Ukrainian).

Ryzhenko, H. F., Horbatiuk, O. I., Andriiashchuk, V. O., Zhovnir, O. V. (2016). [Microbiological contamination of milk and dairy products in subclinical mastitis in cows and ways to prevent them]. Veterinary biotechnology, 29, 233–241.

Founou, L. L. (2016). Antibiotic Resistance in the Food Chain: A Developing Country-Perspective. Front Microbiol. 7, 1881. https://doi.org/10.3389/fmicb.2016.01881

Korotkevych, Yu. V. (2016). [Analysis of antibiotic resistance of enterobacteria and enterococci isolated from food]. Voprosy pitanija. – Nutrition issues, 85(2), 5-13.

Euro-Asian Council for Standardization, Metrology and Certification (2009). [Milk and dairy products. Microbiological analysis methods]. (GOST 9225-84). Мoskow, Standartinform (in Russian).

Euro-Asian Council for Standardization, Metrology and Certification (2016). [Milk and dairy products. Methods for the determination of Staphylococcus aureus]. (GOST 30347-2016). Мoskow, Standartinform (in Russian).

State Committee for Technical Regulation and Consumer Policy of Ukraine. (2003). [Microbiology of food and animal feed. Preparation of test samples, initial suspension and ten-fold dilutions for microbiological examination. Part 1. General rules for preparation of the initial suspension and ten-fold dilutions]. (DSTU ISO 6887-1:2003). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).

Federal Agency on Technical Regulating and Metrology (2011). [Meat and meat products. General requirements and methods of microbiological analysis]. (GOST R Р 54354-2011). Мoskow, Standartinform (in Russian).

Jejdel'shtejn, M.V. (2001) Vyjavlenie β-laktamaz rasshirennogo spektra u gramotricatel'nyh bakterij s pomoshh'ju fenotipicheskih metodov. Klinicheskaja mikrobiologija i antimikrobnaja terapija, 3(2), 183-189 (in Russian).

Marimuthu, K., & Harbarth, S. (2014). Screening for methicillin-resistant Staphylococcus aureus. Current Opinion in Infectious Diseases, 27(4), 356–362. https://doi.org/10.1097/QCO.0000000000000081

Gostev, V. V., Kalinogorskaja, O. S., Popenko, L. N., Chernen'kaja, T. V., Naumenko, Z. S., Voroshilova, T. M., Zaharova, J. A., Hohlova, O. E., Kruglov, A. N., Ershova, M. G., Molchanova, I. V., & Sidorenk, S. V. (2015). Antibiotikorezistentnost' meticillinorezistentnyh Staphylococcus aureus, cirkulirujushhih v Rossijskoj Federacii. Antibiotiki i Himioterapija, 60(1-2), 3–9 (in Russian).

Khan, A., Wilson, B., & Gould, I. (2018). Current and future treatment options for community-associated MRSA infection. Expert Opinion on Pharmacotherapy, 19(5), 457–470.

doi: 10.1080/14656566.2018.1442826

Zheng, X., Berti, A., McCrone, S., Roch, M., Rosato, A., Rose, W., & Chen B. (2018). Combination antibiotic exposure selectively alters the development of vancomycin intermediate resistance in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 62(2), 10-17. https://doi.org/10.1128/AAC.02100-17

Rybak, J., Marx, K., & Martin, C. (2014). Early experience with tedizolid: Clinical efficacy, pharmacodynamics, and resistance. Pharmacotherapy, 34(11), 1198–1208. https://doi.org/10.1002/phar.1491

Oh, E., Bae, J., Kumar, A., Choi, H., & Jeon, B. (2018). Antioxidant-based synergistic eradication of methicillin-resistant Staphylococcus aureus (MRSA) biofilms with bacitracin. International Journal of Antimicrobial Agents. 52(1), 96-99 https://doi.org/10.1016/j.ijantimicag.2018.03.006

Para, R., Fomda, B., Jan, R., Shah, S., & Koul, P. (2018). Microbial etiology in hospitalized North Indian adults with community-acquired pneumonia. Lung India: Official Organ of Indian Chest Society, 35(2), 108–115. https://doi.org/10.4103/lungindia.lungindia_288_17

Magiorakos, A.-P., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske, C. G., Harbarth, S., Hindler, J. F., Kahlmeter, G., Olsson-Liljequist, B., Paterson, D. L., Rice, L. B., Stelling, J., Struelens, M. J., Vatopoulos, A., Weber, J. T. Monnet D. L. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3), 268–281. https://doi.org/10.1111/j.1469-0691.2011.03570.x.

Minaeva, L. P. (2017). [The safety problems of meat and products from it when they are contaminated with antibiotics]. Voprosy dietologii – Nutritional Issues. 7(4), 31–34.

Tatarnikova, N. A. (2014). [Antibiotics in food]. Izvestija OGAU. 5(49), 208-211.

Merezhko, O. E. (2015). [The formation of the resistance of microorganisms when antibiotics are added to feed]. Izvestija OGAU. 2 (52). 174–176.

Mahami T., Togby-Tetteh W., Kottoh D., Amoakoah-Twum L. (2019). Microbial food safety risk to humans associated with poultry feed: the role of irradiation. International journal of food science. https://doi.org/10.1155/2019/6915736

Sanjit S. A., Lekshmi M., Prakasan S., Nayak B. B., Kumar S. (2017). Multiple antibiotic-resistant, extended spectrum-β-Lactamase (ESBL)-producing enterobacteria in fresh seafood. Microorganisms, 5(3). 30. https://doi.org/10.3390/microorganisms5030053

European Committee on Antimicrobial Susceptibility Testing et.al. Breakpoint tables for interpretation of MICs and zonediameters. Version 5.0, January. 2015.

Igbinosa, E. O., Beshiru, A., Akporehe, L. U., Ogofure, A. G. (2016) Detection of methicillin-resistant Staphylococci isolated from food producing animals: A public health implication. Vet Sci. 3(3). 14. https://doi.org/10.3390/vetsci3030014

Dweba, C. C., Zishiri, O. T., El Zowalaty, M. E. (2018). Methicillin-resistant Staphylococcus aureus: livestock-associated, antimicrobial, and heavy metal resistance..Infect Drug Resist. 11, 2497–2509. https://doi.org/10.2147/IDR.S175967

Polyvanov, Ye. A., Kondratiuk, N. V., Viienko, O. Yu., Honcharenko, I. P. (2020). [Theoretical aspects of creating food hydrogels with glucuronic acid]. Visnyk NTU «KhPI», Seriia: Innovatsiini doslidzhennia u naukovykh robotakh studentiv, 5(1359), 86–91. https://doi.org/10.20998/2220-4784.2020.05.13






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