ISOLATION OF LYSOZYME OF BLACK SEA MUSSEL MUTILUS GALLOPROVINCIALIS

Abstract

The analytical review showed the promising use of lysozyme as a valuable natural antibacterial drug. The eperimental monitoring of natural sources of drugs with antibacterial activity showed the feasibility of using secondary raw materials from water resources of the Black Sea basin, obtained during the mussels processing. The isolation and chromatographic purification of lysozyme was carried out. Only the samples eluted with 0.6 M NaCl solution in 0.02 M acetate buffer, pH 5.0, were found out to exhibit lytic activity. The fraction possessinglytic activity was 54 cm³ and corresponded to the elution with 0.6 M solution of NaCl in 0.02 M acetate buffer, pH 5.0. The lysozyme under study, according to the results of gel electrophoresis in 15% PAH using the calculated calibration curve,  is characterized by a molecular weight of 18 kDa, which confirms its belonging to low molecular weight proteins. A preparation of lysozyme with a specific activity of 206 u/mg with a molecular weight of 18 kDa and purification degree of 946.6 was obtained from the juice of Black Sea mussels. The antibacterial activity of the obtained lysozyme preparation against pathogens of food poisoning and food spoilage was studied. The data obtained allow us to predict the prospects of using mussels as a source of highly active lysozyme.