• Oleg I. Yurchenko V.N. Karazin Kharkiv National University, Ukraine
  • Tetyana V. Chernozhuk V. N. Karazin Kharkiv National University, Ukraine
  • Oleksii A. Kravchenko V. N. Karazin Kharkov National University, Ukraine
  • Аlexandr N. Baklanov V.N. Karazin Kharkiv National University, Ukraine



soil, sample preparation, ultrasound, Triton X-100, atomic absorption spectroscopyю, atomic emission spectroscopy with inductive coupled plasma, X-ray fluorescent analysis, Copper, Zinc, acetylacetonates of Copper and Zinc.


The optimal conditions for the flame atomic absorption determination of Zinc and Copper are found. The optimal concentration of Triton X-100 was 4%, and the sonication time was 15 min. The results of the determination were reliably confirmed by X-ray fluorescence and atomic emission analysis methods with inductively coupled plasma. The use of non-ionic surfactant allowed to reduce the surface tension of the analyzed solution, increase its dispersion and the reducing properties of the flame. This made it possible to increase the sensitivity of the atomic absorption determination of copper by 1.5 times and zinc by 1.6 times. Soil sample preparation was performed using ultrasound and surfactants. The content of analytes in the soil was determined using acetylacetones of copper and zinc as standard samples of the composition, which significantly increased the precision and accuracy of metal determination. By varying the mass of the sample and the "added – found" method, it is shown that the systematic error is insignificant.

Author Biography

Аlexandr N. Baklanov, V.N. Karazin Kharkiv National University

професор кафедри освтніх технологій та охорони праці Української інженерно-педагогічної академії 1,0 ставки

професор кафедри хімічної метрології Харківського національного університету імені В.Н. Каразіна 0,25 ставки


Houda, P. S. (Ed.). (2010). Trace elements in soils. Chichester, Great Britain: J. Wiley & Sons.

Magnani, A., Ajmone-Marsan, F., D’Amico, M., Balestrini, R., Viviano, G., Salerno, F., Freppaz, M. (2018). Soil properties and trace elements distribution along an altitudinal gradient on the southern slope of Mt. Everest, Nepal. Catena 162, 61–71.

Addis, W., Abebaw, A., Pashikanti, S. (2017). Determination of heavy metal concentration in soils used for cultivation of Allium sativum L. in East Gojjam Zone, Amhara Region, Ethiopia. Cogent Chem. 3 (1), 1419422.

Anacletus, F. C., Nwauche, K. T., Ighorodje-Monago, C. C. (2017). Mineral and heavy metal composition of crude oil polluted soil amended with non-ionic surfactant (Triton X-100) and white rot fungus (Pleurotus ostratus). J. Environ. Anal. Toxicol. 7(3), 449–451.

Singh, V., Padalia, D., Devlal, K. (2017). Determination of Cu, Zn, Mn and Fe Metals in Soil Employing the EDXRF and FAAS Techniques and Comparative Study of Results. J. Nucl. Phys. Mater. Sci. Radiation Applications 4(2), 383–391.

Lagoida, I. A., Trushin, A. V. (2016). X-ray fluorescence analysis of low concentrations metals in geological samples and technological products. J. Phys. Conf. Ser. 675(4), 042002.

Li, F., Lu, A., Wang, J. (2017). Modeling of chromium, copper, zinc, arsenic and lead using portable X-ray fluorescence spectrometer based on discrete wavelet transform. J. Environ. Res. Public Health. 14(10), 1163.

Moller, K.M., Hartwell, J. G., Simon-Friedt, B. R., Wilson, M. J., Wickliffe, J. K. (2018). Soil contaminant concentrations at urban agricultural sites in New Orleans, Louisiana: A comparison of two analytical methods. J. Agric. Food. Systems Community Development 8(2), 139–149.

Rehan, I., Gondal, M. A., Rehan, K. (2018). Determination of lead content in drilling fueled soil using laser induced spectral analysis and its cross validation using ICP/OES method. Talanta 182, 443–449.

Nyika, J., Onyari, E., Dinka, M. O., Mishra, S. B. (2020). Comparison of reproducibility of inductively coupled spectrometric techniques in soil metal analyses. Air, Soil Water Res. 12, 1–9.

Mao, J.-C., Liu, X.-Y., Chen, B., Luo, F.-M., Wu, X.-D., (2017). Determination of heavy metals in soil by inductively coupled plasma mass spectrometer (ICP-MS) with internal standard method. Electronics Sci. Technology and Application. 4 (1), 23–31.

Hurdebise, Q., Tarayre, C., Fisher, C., Colinet, G., Hiligsmann, S., Delvigne, F. (2015). Determination of zinc, cadmium and lead bioavailability in contaminated soil at the single-cell level by a combination of whole-cell biosensors and flow cytometry. Sensors. 15, 8981–8999.

Bamrah, R. K., Vijayan, P., Karunakaran, C., Muir, D., Hallin, E., Stobbs, J., Goetz, B., Nickerson, M., Tanino, K., Warkentin, T.D. (2019). Evaluation of X-ray fluorescence spectroscopy as a tool for nutrient analysis of Pea seeds. Crop Sci. 59, 1–12.

Kazantzi, V., Drosaki, E., Skok, A., Vishnikin, A.B., Anthemidis, A. (2019). Evaluation of polypropylene and polyethylene as sorbent packing materials in on-line preconcentration columns for trace Pb(II) and Cd(II) determination by FAAS. Microchem J. 148, 514–520.

Vishnikin, A., Miekh, Yu., Denisenko, T., Bazel, Ya., Andruch, V. (2018). Use of sequential injection analysis with lab-at-valve and optical probe for simultaneous spectrophotometric determination of ascorbic acid and cysteine by mean centering of ratio kinetic profiles. Talanta 188, 99–106.

Al-Shwaiyat, M.K.E.A., Miekh, Y.V., Denisenko, T.A., Vishnikin, A.B., Andruch, V., Bazel, Ya.R. (2018). Simultaneous determination of rutin and ascorbic acid in a sequential injection lab-at-valve system. J. Pharm. Biomed. Anal. 149, 179–184.

Goloperov I. V., Belova E. A., Baklanova L. V., Baklanov A. N. (2018). Improving food safety – increase of expressive analysis to toxic elements. ISJ Theoretical & Applied Science, 57, (1), 260–265.

Yurchenko O. I., Chernozhuk Т. V., Baklanov A. N., Baklanova L. V., Kravchenko O. A. (2018). Analytical signal amplification technologies in sonoluminescence spectroscopy by double-frequency ultrasound. Methods Objects Chem. Anal. 13 (3), 103–109.

Yurchenko, O.I., Kalinenko, O.S., Baklanov, A.N., Belov, E.A., Baklanova, L.V. (2016). Sonoluminescence spectroscopy as a promising new analytical method. J. Appl. Spectrosc. 83, 105–110.

Chmilenko, F.A., Baklanov, A.N., Sidorova, L.P., Lebedeva, E.V., Lebedeva, A.V. (2001). Ultrasonic intensification of sample preparation for the spectrophotometric determination of arsenic in foodstuffs. J. Anal. Chem. 56, 13–16.

Baklanov, A.N., Chmilenko, F.A. (2001). Use of ultrasound in sample preparation for the determination of mercury species by cold-vapor atomic absorption spectrometry. J. Anal. Chem. 56, 641–646.

Yurchenko, O.I., Chernozhuk, T.V., Baklanov, A.N., Kravchenko, O.A. (2021). Analysis of water and bottom sediments of the Tiger river (Iraq) using ultrasonic treatment, nonionic surface active substances and β-diketonates of metals as standard samples. J. Chem. Technologies 29 (2), 173–178.