INNOVATIVE APPROACHES TO THE DETERMINATION OF FRUCTOSE IN DAIRY PRODUCTS WITH COMBINED CARBOHYDRATES

Authors

DOI:

https://doi.org/10.15421/jchemtech.v30i2.236760

Keywords:

dairy products, carbohydrate composition, fructose content, destruction of lactose, resorcinol method, method accuracy, reliability of results

Abstract

Determination of carbohydrate content in food products, in particular dairy products, is necessary to solve various applications, including quality control. There are currently no techniques or methods for determining fructose in dairy products with a combined carbohydrate composition, including for baby food. Given the multicomponent composition of dairy products for baby food, there is a need to separate carbohydrates, in particular fructose, from the components included in the matrices of the analyzed sample and create certain difficulties for its quantification. The aim was to apply innovative approaches to develop a method of extraction and use of the resorcinol method for the determination of fructose in dairy products with a combined carbohydrate composition, including for baby food. The founded that according to the absorption spectra of the filtrate reaction products obtained by various methods, the maximum absorption in the visible region of the spectrum is  λmax = 415-420 nm. It was found that the value of the mass fraction of fructose in the filtrates obtained by precipitation of the product solutions for the polarimetric method with the destruction of lactose, correlate with each other, namely, the value of the absolute error of the compared results is 0.02 and 0.03%, and the relative error is 0.6-0.73%. An innovative method for extracting fructose in dairy products with a combined carbohydrate composition, including for baby food, to determine its resorcinol method, proved the accuracy of the method and the reliability of the results in the filtrates obtained by precipitation of the product solutions for polarimetric method with lactose destruction.

References

Gray, J. (2003). Carbohydrates: nutritional and health aspects. ILSI Press, Europe.

Polumbryk, M. O. (2011). [Carbohydrates in food and human health]. Kyiv, Ukraine: Akademperiodyka (in Ukrainian).

Introduction of complementary foods when feeding children of the first year of life. https://uda.in.ua/25-02-2016-р-публікація-тижня-введення-прикор/

Ladodo, K. S. (2008). [Rational nutrition of young children]. Moskow, Russian Federation: Miklos (in Russian).

Romanchuk, I. O., Kihel, N. F., Rudakova, T. V., Danylenko, S. H., Andreus, S. M., Moiseeva, L. O. Ukraine Patent No 104976. Vitalakt sour-milk product. Kyiv, Ukraine. Institute of Food Resources (in Ukrainian).

Romanchuk, I. O., Rudakova, T. V., Andreus, S. M., Moiseeva, L. O. Ukraine Patent No 105581. Cheese paste for baby food. Kyiv, Ukraine. Institute of Food Resources (in Ukrainian).

Petrukhin, O. M. (1992). [Analytical chemistry. Chemical methods of analysis]. Moskow, Russian Federation: Khimiya (in Russian).

Haritonov, Ju. Ja. (2018). [Analytical chemistry: textbook]. Moscow, Russian Federation: GEOTAR-Media (in Russian).

Pedruzzi, I. (2007). Quantification of lactobionic acid and sorbitol from enzymatic reaction of fructose and lactose by high-performance liquid chromatography. J. Chromatogr. A. 1145, 1-2, 128–132.

Rudakov, O. B. (2005). [Modern liquid chromatography of carbohydrates]. Oils and fats. 58, 12, 13–14 (in Russian).

Slimestad, R. (2006). Thermal stability of glucose and other sugar aldoses in normal phase high performance liquid chromatography. J. Chromatogr. A. 1118(2), 281–284.

Kirhner, Ju. (1981). [Thin layer chromatography]. Moskow, Russian Federation: Mir (in Russian).

Determination of carbohydrates in infant milk powders by ultra-performance liquid chromatography with evaporative light scattering detector and BEH HILIC column. https://www.researchgate.net/publication/321210387_Determination_of_carbohydrates_in_infant_milk_powders_by_ultraperformance_liquid_chromatography_with_evaporative_light_scattering_detector_and_BEH_HILIC_column

Analysis of mono- and disaccharides in milk-based formulae by high-performance liquid chromatography with refractive index detection. https://www.researchgate.net/publication/ 8382295_Analysis_of_monoand_disaccharides_in_milk-based_formulae_by_high-performance_liquid_chromatography_with_refractive_index_detection.

Bordak, L. V. (2011). [Gravimetric method for determining fiber in baby food]. Health and environment, 19, 396–401 (in Russian).

Volkova, O. P., Ruvinskij, O. E., Frampol'skaja, T. V. (2000). [Express method for potentiometric determination of fructose in fermented milk drinks with Jerusalem artichoke]. Proceedings of universities, Food technology, 5-6, 78–80. (in Russian).

Pisarev, D. I., Novikov, O. O., Bezmenova, M. D., Tomchakovskaja, E. A., Sorokopudova, V. N. (2010). [Development of a method for the quantitative determination of monosaccharides in late bird cherry fruits. Scientific statements]. Ser. The medicine. Pharmacy. 93(22), 114–118 [in Russian].

Steegmans, M., Iliaens, S., Hoebregs, H. (2004). Enzymatic, Spectrophotometric Determination of Glucose, Fructose, Sucrose, and Inulin/Oligofructose in Foods. Journal of AOAC International, 87(5), 1200–1207.

Krus', G. N., Shalygina, A. M., Volokitin, Z. V. (2002). [Research methods of milk and dairy products]. Moscow, Russian Federation: KolosS (in Russian).

Inihov, G. S., Brio, N. P. (1971). [Methods for the analysis of milk and dairy products]. Moscow, Russian Federation: Pishchevaya promyshlennost' (in Russian).

Prasad, L. Polavarapu (2002). Optical rotation: Recent advances in determining the absolute configuration. Chirality, 10, 768–781.

Lisicin, V. M., Antonenko, E. S., Velichko, D. I. (1984). [Solving engineering and economic problems on a computer]. Kiev, Ukraine: Vyshcha shkola.

Aleksandrov, Ju. I., Beljakov, V. I. (2002). [Uncertainty and uncertainty of the chemical analysis result]. Journal of Analytical Chemistry, 57(2), 118–129 (in Russian).

Bochkov, A. F., Afanas'ev, V. A., Zaikov, G. E. (1980). [Carbohydrates]. Moscow, Russian Federation: Nauka (in Russian).

Korenman, Ja. I., Mokshina, N. Ja., Bychkova, A. A. (2014). [Physicochemical determination of carbohydrates in food and beverages]. Vestnik VSUIT. 59(1), 146-152 (in Russian).

Shahidullah, M., Khorasani, S. S. M. A. (1972). The sensitivity and selectivity of the Seliwanoff test for fructose. Analytica Chimica Acta, 61, 317–319.

Roe, J. H. (1934). A colorimetric method for the determination of fructose in blood and urine. The Journal of Biological Chemistry, 107, 15–22.

Published

2022-07-25