INNOVATIVE APPROACHES TO THE DETERMINATION OF FRUCTOSE IN DAIRY PRODUCTS WITH COMBINED CARBOHYDRATES
Keywords:dairy products, carbohydrate composition, fructose content, destruction of lactose, resorcinol method, method accuracy, reliability of results
Determination of carbohydrate content in food products, in particular dairy products, is necessary to solve various applications, including quality control. There are currently no techniques or methods for determining fructose in dairy products with a combined carbohydrate composition, including for baby food. Given the multicomponent composition of dairy products for baby food, there is a need to separate carbohydrates, in particular fructose, from the components included in the matrices of the analyzed sample and create certain difficulties for its quantification. The aim was to apply innovative approaches to develop a method of extraction and use of the resorcinol method for the determination of fructose in dairy products with a combined carbohydrate composition, including for baby food. The founded that according to the absorption spectra of the filtrate reaction products obtained by various methods, the maximum absorption in the visible region of the spectrum is λmax = 415-420 nm. It was found that the value of the mass fraction of fructose in the filtrates obtained by precipitation of the product solutions for the polarimetric method with the destruction of lactose, correlate with each other, namely, the value of the absolute error of the compared results is 0.02 and 0.03%, and the relative error is 0.6-0.73%. An innovative method for extracting fructose in dairy products with a combined carbohydrate composition, including for baby food, to determine its resorcinol method, proved the accuracy of the method and the reliability of the results in the filtrates obtained by precipitation of the product solutions for polarimetric method with lactose destruction.
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