IR spectra, gel systems, agar, glycerin, honey


The purpose of this work was to determine the effect of glycerin TM BASF, sunflower honey and sesame powder TM "Korysne Boroshno" on the state of water in gel systems based on agar 1200 TM "Fujian Province" for the production of jelly bars. The research was carried out using infrared spectroscopy on a Perkin-Elmer Spectrum One FTIR Spectrometer. The study showed that gel formation in the "agar-water-glycerol" system leads to redistribution of OH groups, an increase in the number of associated hydroxyls, and water adsorption. This is confirmed by the appearance of a characteristic broad intense absorption band in the region of 37002600 cm–1 in the IR spectrum. The addition of honey to the gel system leads to a change in the intensity of the band of valence vibrations of the С–С bond in honey carbohydrates (at 1245 cm–1) and its shift towards higher frequencies compared to the "agar-water-glycerol" gel, which is confirmed by the presence of a band absorption in the region of 1200–700 cm–1. Spectroscopy data also showed that the addition of sesame powder during the preparation of an agar-based gel reduced the intensity of the OH valence vibrations of the water group (2151 cm–1), and also led to a shift of the intense broad band towards higher frequencies compared to gels without sesame (2139 cm–1).


Bokovets, S., Pertsevoi, F., Melnyk, O., Gurskyi, P., Marenkova, T. (2020). [Features of the new chocolate bar technology]. Prohresyvni tekhnika ta tekhnolohii kharchovykh vyrobnytstv restorannoho hospodarstva i torhivli, 2(32), 32-41 (in Ukrainian).

Vagner De Alencar Arnaut De Toledo, Emerson Dechechi Chambo. (2020). Honey Analysis: New Advances and Challenges. London, UK: BoD – Books on Demand.

Eva, C. (2019). A Book of Honey. UK: Northern Bee Books

Victor, R. P. (Eds.). (2014). Processing and Impact on Active Components in Food. USA: Academic Press.

Joseph, F. Z. (2012). Functionality of Proteins in Food. Heidelberg, Germany: Springer Science & Business Media.

Gubsky, S. M., Muzyka, S. M., Foshan, A. L., Evlash, V. V., Kalugin, O. N. (2018). Reologic properties of aqueous solutions of agar and gelatine for confectionery. Kharkiv University Bulletin. Chemical Series, (31), 64–78.

Martinez-Sanz, M., Gomez-Mascaraque, L. G., Ballester, A. R., Martinez-Abad, A., Brodkorb, A., Lopez-Rubio, A. (2019). Production of unpurified agar-based extracts from red seaweed Gelidium sesquipedale by means of simplified extraction protocols. Algal Res., 38, 101420.

Roy, S, Rhim, J. W. (2019). Agar-based antioxidant composite films incorporated with melanin nanoparticles. Food Hydrocoll., 94, 391–398.

Laaman, T.R. (2011). Hydrocolloids in food processing. Oxford, UK: Wiley-Blackwell.

Nieto, M. B., Akins, M. (2010). Hydrocolloids in bakery fillings. Hydrocolloids in food processing. In T. R. Laaman (Ed.). USA: WileyBlackwell Publishing Ltd.

Barbara, H. S. (2004). Infrared Spectroscopy: Fundamentals and Applications. Sydney, Australia: John Wiley & Sons.

Osborne, B. G., Fearn, T. (1988). Near-Infrared Spectroscopy in Food Analysis. New York, USA: Wiley.

Madera-Santana, T. J., Freile-Pelegrin, Y., Azamar-Barrios, J. A. (2014). Physicochemical and morphological properties of plasticized poly(vinyl alcohol)-agar biodegradable films. Int. J. Biol. Macromol., 69, 176–184.

Alpaslan, D., Dudu, T. E., Aktas, N. (2021). Synthesis and characterization of novel organo-hydrogel based agar, glycerol and peppermint oil as a natural drug carrier/release material. Mater. Sci. Eng. C., 118, 111534.

Sinaga, M. Z. E., Gea, S., Zuhra, C. F., Sihombing, Y. A., Zaidar, E., Sebayang, F., Ningsih, T. U. (2021). The effect of plasticizers and chitosan concentration on the structure and properties of Gracilaria sp.-based thin films for food packaging purpose. Polimery, 66(2),

Fathiraja, P., Gopalrajan, S., Karunanithi, M., Nagarajan, M., Obaiah, M. C., Durairaj, S., Neethirajan, N. (2022). Response surface methodology model to optimize concentration of agar, alginate and carrageenan for the improved properties of biopolymer film. Polym. Bull., 79, 6211–6237.

Elhefian, E. A., Nasef, M. M., Yahaya, A. H. (2012). Preparation and characterization of chitosan/agar blended films: part 2. Thermal, mechanical, and surface properties. E-J. Chem., 9(2), 510–516.

Fathiraja, P., Gopalrajan, S., Karunanithi, M., Nagarajan, M., Obaiah, M. C., Durairaj, S., Neethirajan, N. (2021). Development of a biodegradable composite film from chitosan, agar and glycerol based on optimization process by response surface methodology. Cellul. Chem. Technol., 55(7–8), 849–865.

Campa-Siqueiros, P. I., Vargas-Arispuro, I., Quintana-Owen, P., Freile-Pelegrin, Y., Azamar-Barrios, J. A., Madera-Santana, T. J. (2020). Physicochemical and transport properties of biodegradable agar films impregnated with natural semiochemical based-on hydroalcoholic garlic extract. Int. J. Biol. Macromol., 151, 27–35.

Wu, Y., Geng, F., Chang, P. R., Yu, J., Ma, X. (2009). Effect of agar on the microstructure and performance of potato starch film. Carbohydr. Polym., 76, 299–304.

Phillips, G., Williams, P. (Eds.). (2009). Handbook of Hydrocolloids. Second Edition. Woodhead Publishing Limited : CRC Press.

Elhefian, E., Nasef, M., Yahaya, A. (2012). Preparation and Characterization of Chitosan/Agar Blended Films: Part 1. Chemical Structure and Morphology. J. Chem., 9, 1431–1439.

Rochas, C., Lahaye, M., Yaphe, W. (1986). Sulfate content of carrageenan and agar determined by infrared spectroscopy. Bot. Mar., 29, 335–340.

Shahnaz, L., Shehnaz, H., Haider, A. (2019). Fourier transform infrared (FT-IR) spectroscopic investigations of four agarophytes from northern Arabian sea. Bangladesh J. Bot., 48, 925–932.

Kataoka, Y., Kitadai, N., Hisatomi, O. (2011). Nakashima S. Nature of hydrogen bonding of water molecules in aqueous solutions of glycerol by attenuated total reflection (ATR) infrared spectroscopy. Appl Spectrosc., 65(4), 436–441.

Tykhonov, O. I., Tykhonova, S. O., Yarnykh, T. H., Shpychak, O. S., Podorozhna, L. N., Zuikina, S. S., Andrieieva, I. V., Bohutskaia, Y. I. (2010). [Natural honey in medicine and pharmacy (origin, properties, application, medicinal preparations)]. Kharkiv, Ukraine (in Ukrainian).

Formosa, J. P., Lia, F., Mifsud, D., Farrugia, C. (2020). Application of ATR-FT-MIR for Tracing the Geographical Origin of Honey Produced in the Maltese Islands. Foods, 9, 710.

Sahlan, M., Karwita, S., Gozan, M., Hermansyah, H., Yohda, M., Young, J., Yoo, Y. J., Pratami, D. K. (2019). Identification and classification of honey's authenticity by attenuated total reflectance Fourier-transform infrared spectroscopy and chemometric method. Veterinary World, 12(8), 1304–1310.

Wei, X., Liu, K., Zhang, Y., Feng, Q., Wang, L., Zhao, Y., Li, D., Zhao, Q., Zhu, X., Li, W., Fan, D., Gao, Y., Lu, Y., Zhang, X., Tang, X., Zhou, C., Zhu, C., Liu, L., Zhong, R., Tian, Q., Wen, Z., Weng, Q., Han, B., Huang, X., Zhang, X. (2015). Genetic discovery for oil production and quality in sesame. Nat. Commun., 6, 8609.

Pathak, N., Rai, A., Saha, S., Walia, S., Sen, S., Bhat, K. (2014). Quantitative dissection of antioxidative bioactive components in cultivated and wild sesame germplasm reveals potentially exploitable wide genetic variability. J. Crop Sci. Biotechnol., 17, 127-139.

Safonova, O., Teymurova, A. (2011). Infrared spectroscopy study of water in protein-polysaccharide gels. Printed Proceedings of 11th International Congress on Engineering and Food (ICEF 11), 2, 969.