ISOLATION AND RESEARCH OF MOLECULAR MASS AND COMPOSITION OF LYSOCYM ARMORACIA RUSTICANA BY THE ENZYME-SUBSTRATE CHROMATOGRAPHY METHOD
DOI:
https://doi.org/10.15421/jchemtech.v30i1.255480Keywords:
lysozyme, isolation, Armoracia rusticana root crops, enzyme-substrate chromatography, gel electrophoresisAbstract
The article presents the results of the extraction of plant lysozyme from Armoracia rusticana by specific enzyme-substrate chromatography. A chromatographic curve of lysozyme isolation from the juice part of Armoracia rusticana root crops using as a biospecific sorbent, glucochitin, which is presented. A preparation of lysozyme was obtained from the juice part of the roots of Armoracia rusticana with a specific activity of 217.6 units/mg. The lysozyme, which was examined by gel electrophoresis in 15 % polyacrylamide gel using a calibration curve is characterized by a molecular mass of 12.022 kDa, that confirms that it belongs to low-molecular-weight proteins. The yield of the lysozyme preparation by the total lysozyme activity is 36.8 %. The data obtained allow predicting the prospects of using Armoracia rusticana as a source of plant-based lysozyme.
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