• Larisa P. Sidorova Oles Honchar Dnipro National University, Ukraine
  • Andriy B. Vishnikin Oles Honchar Dnipro National University, Ukraine
  • Maryna G. Sydorova Oles Honchar Dnipro National University, Ukraine



simultaneous determination; synthetic food dyes; E 110 (yellow "Sunset"); E 124 (Ponceau 4R, Bright red 4R); E 102 (Tartrazine), spectrophotometry; Mean Centering method; Ratio difference method; soft drinks.


Two simple spectrophotometric methods have been developed for the simultaneous spectrophotometric determination of synthetic food dyes (SFD) in a binary mixtures using the Mean centering (MC) and Ratio difference (RD) methods. The analytical characteristics of MC and RD methods for determining the content of SFD in mixtures of dye Yellow Sunset (E110) with Ponceau 4R (E124) and Tartrazine (E102) with E124 in the model solutions and carbonated beverages were evaluated. Analytical wavelengths of 560 and 420 nm and 430 and 490 nm for the first (E110 and E124) and second (E102 and E110) mixtures, respectively, were selected for the implementation of methods and construction of calibration graphs. The concentration ranges at which the dyes can be determined are from 1 to 30 μg / mL. For both methods, the correlation coefficients were obtained, the value of which is not less than 0.995, which indicates the absence of influence of the calculation method on the obtained results. It is established that the error of determination of individual dyes by MC and RD methods is at the level of 1-3% in model mixtures with different concentration ratios of components. The percentage of found dye contents varied for E110 from 98.3% to 101.8%, for E124 – from 98.6% to 99.2%, Е102 – from 98.4% to 102.0%, The method has been successfully used to determine dyes in soft drinks. The results obtained did not differ within the error from those obtained by chromatography.


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