propolis, oily extract, technology, quality indexes, phenolic compounds


In this work, we studied the extraction of propolis with oils. For the experiment, we used propolis samples collected in different regions of Ukraine (Kharkiv, Sumy, Poltava, Kyiv, and Dnipropetrovsk regions) and determined their compliance with DSTU 4662:2006 "Propolis". All the samples we collected met the requirements of this standard, and a combined batch of propolis was used to obtain oil extracts. The most common vegetable oils (corn, sunflower, and olive oils) were chosen as extragents, and their quality was confirmed. The technological approach to obtaining oil extracts was based on the data of previous studies; the extraction was carried out at 60C and constant stirring for 1 hour. The obtained oil extracts of propolis were evaluated by appearance, acid and peroxide numbers, density, identification and quantification of phenolic substances. Presence of substances of phenolic and flavonoid structure was confirmed by specific reactions. As a result of the quantitative determination of the TPC in the obtained extracts it was established that the total phenolic content (TPC) in the studied oil extracts is about 4.95±0.15 %, which corresponds to the quantitative content of these biologically active substances in the propolis substance (not less than 25.0 %).


Almuhayawi, M.S. (2020). Propolis as a novel antibacterial agent. Sau di journal of biological sciences, 27(11), 3079–3086.

Arvouet-Grand, A., Vennat, B., Pourrat, A., Legret, P. (1994). Standardization of propolis extract and identification of principal constituents. Journal de pharmacie de Belgique, 49(6), 462–468.

Aziz, S., Akbar, A., Gul, Z., Sadiq, M.B., Achakzai, J.K., Khan, N. A., Ali, I. (2022). Functional potential and chemical profile analysis of propolis oil extracted from propolis of Balochistan. Journal of Food Quality, 2022, 1–10.

Bankova, V. (2005). Recent trends and important developments in propolis research. Evidence-based complementary and alternative medicine, 2(1), 29–32.

Bankova, V., Trusheva, B., Popova, M. (2021). Propolis extraction methods: A review. Journal of Apiculture Research, 60(5), 734–743. doi:10.1093/ecam/neh059

Silva, K., Mathias, F., Dutra, K., Kleinubing, Sch., Dalla Santa HS., Rosa, M., Buriol, L., Torres, Y., Monteiro, M. (2010). Antimicrobial activity from a brazilian propolis oily extract. RECEN-Revista Ciências Exatas e Naturais, 12(2), 327–338.

Finger, D., Machado, C. S., Torres, Y. R., Quináia, Sueli P. A., Thomaz, C. G., Gobbo, A. R., Monteiro, M. C., Ferreira, A. G., Sawaya, A.C.H.F., Eberlin, M. N. (2013). Antifungal bioassay‐guided fractionation of an oil extract of propolis. Journal of Food Quality, 36(5), 291–301.

Ghasemi, F.S., Eshraghi, S.S., Andalibi, F., Hooshyar, H., Kalantar-Neyestanaki, D., Samadi, A., Fatahi-Bafghi, M. (2017). Antibacterial effect of propolis extract in oil against different bacteria. Zahedan Journal of Research in Medical Science, 19(3). doi: 10.5812/zjrms.7225

Hudz, N., Korytniuk, O., Yezerska, O., Motyka, O., Turkina, V., Korytniuk R.S., Wieczorek, P. (2020). Evaluation of the total flavonoid content and antimicrobial activity of the tinctures of propolis of Ukrainian origin. Acta Pol. Pharm. Drug Res., 77(2020), 897–907. doi:10.32383/appdr/131651

Kubiliene, L., Laugaliene, V., Pavilonis, A., Maruska, A., Majiene, D., Barcauskaite, K., Kubilius, R., Kasparaviciene, G., Savickas, A. (2015). Alternative preparation of propolis extracts: comparison of their composition and biological activities. BMC complementary and alternative medicine, 15(1), 1–7. doi: 10.1186/s12906-015-0677-5

Przybyłek, I., & Karpiński, TM (2019). Antibacterial properties of propolis. Molecules, 24(11), 2047. doi: 10.3390/molecules24112047

Pujirahayu, Niken, Ritonga, Halimahtussadiyah, Uslinawaty, Zakiah (2014). Properties and flavonoids content in propolis of some extraction method of raw propolis. Int. J. Pharm. Sci, 6(6), 338–40.

Ramanauskienė, K., Inkėnienė, A.M. (2011). Propolis oil extract: quality analysis and evaluation of its antimicrobial activity. Natural product research, 25(15), 1463–1468. doi: 10.1080/14786419.2010.529440

Ramanauskiene, K., Inkeniene, AM, Savickas, A., Masteikova, R., Brusokas, V. (2009). Analysis of the antimicrobial activity of propolis and lysozyme in semisolid emulsion systems. Acta Pol. Pharm., 66(6), 681–688.

Saeed, F., Ahmad, R.S., Arshad, M.U., Niaz, B., Batool, R., Naz, R., Ansar Rasul Suleria, H. (2016). Propolis that curb lifestyle related disorders: An overview. International Journal of Food Properties, 19(2), 420–437.

Stan, L., Mărghitaş, L.A., Dezmirean, D. (2011). Quality criteria for propolis standardization. Scientific Papers Animal Science and Biotechnologies, 44(2), 137–140.

Tichonow, A.I., Jarnych, T.G., Czernych, W.P., Zypaniec, J.A., Tichonowa S.A. (2006). [Theory and practice production leczniczych preparations propolis]. Wyd Apipol-Farma, Myślenice. (in Polish)

Tikhonov, O.I., Bashura, O.G., Yarnykh, T.G., Tikhonova, S.O., Skrypnik-Tikhonov, R.I. (2013). The problem of development of the direction for creation and manufacture of medicines with bee products in Ukraine. Herald of Pharmacy, (4), 37–42.

Tosi, B., Donini, A., Romagnoli, C., Bruni, A. (1996). Antimicrobial activity of some commercial extracts of propolis prepared with different solvents. Phytotherapy research, 10(4), 335–336.<335::AID-PTR828>3.0.CO;2-7

Wagh, V.D. (2013). Propolis: a wonder bees product and its pharmacological potentials. Advances in Pharmacological and Pharmaceutical Sciences, 2013, 308249.

Wieczorek, P.P., Hudz, N., Yezerska, O., Horčinová-Sedláčková, V., Shanaida, M., Korytniuk, O., Jasicka-Misiak, I. (2022). Chemical Variability and Pharmacological Potential of Propolis as a Source for the Development of New Pharmaceutical Products. Molecules, 27 (5), 1600. doi: 10.3390/molecules27051600

Yarnykh, T., Yuryeva, G., Rukhmakova, O., Buryak, M., Herasymova, I. (2020). Evolution of propolis standardization methods. Ukrainian biopharmaceutical journal, 2(63), 4–13. doi:

Yildirim, Z., Hacievliyagil, S., Kutlu, NO, Aydin, NE, Kurkcuoglu, M., Iraz, M., & Durmaz, R. (2004). Effect of water extract of Turkish propolis he tuberculosis infection in guinea pigs Pharmacological Research, 49(3), 287–292. doi: 10.1016/j.phrs.2003.10.007

State Committee for Technical Regulation and Consumer Policy of Ukraine. (2006). [Propolis] (TU U 4662:2006). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).

(2008). [State Pharmacopoeia of Ukraine / State the enterprise "Naukovoexpertny pharmacopoeial center"]. Kharkiv: State enterprise "Scientific expert pharmacopoeial center". (in Ukrainian).

State Committee for Technical Regulation and Consumer Policy of Ukraine. (2009). [Method of determination of acid number and acidity] (DSTU EN ISO 660:2009). Kyiv, Derzhpozhyvstandart Ukraine [in Ukrainian].

State Committee for Technical Regulation and Consumer Policy of Ukraine. (2006). [Fatty vegetable oils. Method for determination of peroxide number]. (DSTU 4570:2006). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).

State Committee for Technical Regulation and Consumer Policy of Ukraine. (1994). [Vegetable oils. Raw materials and processed products. Quality indicators. Terms and definitions]. (DSTU 2575-94). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).

State Committee for Technical Regulation and Consumer Policy of Ukraine. (2004). [Oils. Sampling methods]. (DSTU 4349:2004). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian)

Escriche, I., Juan-Borrás, M. (2018). Standardizing the analysis of phenolic profile in prop olis. Food Research International, 106, 834–841. doi: 10.1016/j.foodres.2018.01.055

Šuran, J., Cepanec, I., Mašek, T., Radić, B., Radić, S., Tlak Gajger, I., Vlainić, J. (2021). Propolis extract and its bioactive compounds – From traditional that modern extraction technologies. Molecules, 26(10), 2930.

Omirbayeva, A.Y., Datkhayev, U.M., Gladukh, I.V., Iudina, I.V., Makhatov, B.K., Orynbassarova, K.K., Makhatov, Zh. (2016). Antimicrobial activity of Carduus crispus extracts. 7. 2657–2661.

Gladukh, I., Grubnik, I., Kukhtenko, H. (2017). Structural-Mechanical Studies of Phytogel "Zhivitan". Journal of Pharmaceutical Sciences and Research, 9(10), 1672–1676.