FLUORESCENCE-BASED QUANTIFICATION OF PEPTIDE ADSORPTION ON TITANIUM DIOXIDE

Authors

  • Iryna V. Prokipchuk Vasyl Stefanyk Precarpathian National University, Ukraine https://orcid.org/0000-0001-9452-403X
  • Ihor M. Mykytyn Vasyl Stefanyk Precarpathian National University, Ukraine https://orcid.org/0000-0002-5283-6710
  • Dmytro M. Danyliuk Vasyl Stefanyk Precarpathian National University, Ukraine
  • Mariana V. Bedrii Vasyl Stefanyk Precarpathian National University, Ukraine
  • Mariia Ya. Pidhirna Vasyl Stefanyk Precarpathian National University, Ukraine

DOI:

https://doi.org/10.15421/jchemtech.v31i3.284204

Keywords:

titanium dioxide, adsorption, fluorescence, peptide

Abstract

Titanium dioxide is widely used as a white colorant in medicines, cosmetics, and in the food industry due to the absence of taste and smell, chemical stability in combination with excellent ultraviolet protection properties. However, its safety as a food additive was recently questioned by the European Food Safety Authority (EFSA). There is a need to deeper study the interaction of TiO2 with the human body and biological tissues. Proteins are the main type of biological molecules that can interact with TiO2 after oral administration of TiO2-containing drugs. The classical approach to measuring adsorption of organic molecules on TiO2 is based on spectrophotometry. However, the sensitivity of this method is not sufficient for measurements of the adsorption of peptides and proteins. This problem can be bypassed using peptides covalently labeled with fluorescent organic dyes possessing relatively high molar absorption coefficients and long absorption wavelengths. In this work, we decided to test the applicability of fluorescence-based quantification of peptide adsorption on titanium dioxide. Namely, we used 11 amino acids peptide labeled with fluorescein and quantified its adsorption on TiO2 at different ionic strengths of the solution. There are several advantages of using the fluorescence method to study the binding of peptides to a sorbent surface. The use of low peptide concentrations and small sample volumes allows for efficient use of resources. Reliable readout, fast measurement time, and cost-effectiveness make this method attractive for the future peptide binding studies and could potentially find application in other areas of peptide research.

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Published

2023-10-28

Issue

Vol. 31 No. 3 (2023): Journal of Chemistry and Technologies

Section

Physical and inorganic chemistry

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