BIOACTIVE COMPOUNDS EXTRACTED FROM THE HERB SPERANSKIA TUBERCULATA (BUNGE) BAILL
DOI:
https://doi.org/10.15421/jchemtech.v32i3.305125Keywords:
Speranskia tuberculata (Bunge) Baill; ethyl acetate extract; petroleum ether extract; LC/MS; commercially available cancer cell lines; MTT cytotoxicity test.Abstract
Aim. Identification of bioactive compounds in preparations extracted from the herb Speranskia tuberculata using two different solvents and comparison of the effects of the extracts on human cancer cells of four different lines. Methods. Crude extracts were extracted using ethyl acetate or petroleum ether, followed by distillation, drying and preparation of working solutions based on dimethyl sulfoxide in the 12.5–75 μg/mL concentration range. MTT tests were used to determine the effect of drug concentrations on the viability of cancer cells of four commercially available lines: A549 (lung adenocarcinoma), HEPG2 (hepatocellular carcinoma of the liver), A375 (malignant melanoma) and HELA (pancreatic carcinoma). The tandem liquid chromatography-mass spectroscopy (LC/MS) method was used to identify the main compounds and compare the composition of both extracts. Results. The developed preparations reduced the viability of all 4 types of cancer cells. Ethyl acetate extracts are always more effective than petroleum ether extracts. IC50 of ethyl acetate extract varied between 49–53 μg/mL for all cell lines. Approximately 100–200 compounds have been identified, and their number in petroleum ether extracts is roughly twice as high. Concentrations of 11 compounds significantly increased when switching from extraction with petroleum ether to ethyl acetate. The potential biological activity of identified compounds was analysed based on literature data. Conclusions. The studied preparations reduced the viability of various human cancer cells. Preparations extracted with ethyl acetate were more effective against all types of cells. Hundreds of compounds were identified in the extracts; some anticancer effects were discussed.
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