SYNTHESIS OF SILVER NANOPARTICLES AND THEIR CONJUGATE WITH CEFTRIAXONE USING CHAENOMELES JAPONICA, CHARACTERIZATION, AND ACTIVITY AGAINST STAPHYLOCOCCUS EPIDERMIDIS
DOI:
https://doi.org/10.15421/jchemtech.v33i1.311700Keywords:
green synthesis, AgNPs, UV-Vis, FTIR, SEM, SERS, bacterial resistance, ceftriaxone-conjugated nanoparticlesAbstract
Silver nanoparticles (AgNPs) synthesized on the base of biological matrices are being actively studied, especially as antimicrobial agents against resistant pathogens. The paper reports the biosynthesis of AgNPs using Chaenomeles japonica aqueous leaf extract. Production of Ch-AgNPs and ceftriaxone-conjugated Ch-AgNPs-Cfx was confirmed by UV-Vis spectroscopy with the surface Plasmon resonance peaks at 472 and 475 nm respectively. SEM micrographs showed the fabricated AgNPs with a round, nearly spherical shape, and an average size of 30 – 35 nm. Fourier transform infrared (FTIR) spectroscopy designated the involvement of hydroxyl and carboxyl functional groups of phenolics, flavonoids, terpenoids, alcohols, and carboxylic acids from Ch. japonica extract into bioreduction process of Ag+ to Ag0, and participation of protein carbonyl and amine groups in the capping and stabilization of AgNPs, as well as the binding of β-lactam ring of ceftriaxone with Ch-AgNPs-Cfx. Raman spectroscopy of Ch-AgNPs detected the significant SERS effect for Rhodamine 6G registered at 10-7 M, which confirmed the suitability of phytosynthesized AgNPs as the effective substrates in development of new biosensors. Antibacterial activity of Ch-AgNPs and Ch-AgNPs-Cfx against Staphylococcus epidermidis clinical strain, resistant to several common cephalosporins, was dose-dependent (in the range 2.5–20.0 μg/disc) in the absence of ceftriaxone antibacterial activity, indicating the potential ability of both biosynthesized nanomaterials to overcome the antibiotic resistance of St. epidermidis. Further research is needed to confirm the applicability of Ch-AgNPs and Ch-AgNPs-Cfx for development the new antibacterial drugs against the resistant bacterial strains.
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