SPECTROPHOTOMETRIC DETERMINATION OF THIAMINE USING OXIDATION REACTION WITH 18-MOLYBDODIPHOSPHATE
DOI:
https://doi.org/10.15421/jchemtech.v33i1.321291Keywords:
spectrophotometry, thiamine, 18-molybdodiphosphate, pharmaceuticalsAbstract
A simple and highly sensitive method for the determination of thiamine in pharmaceuticals has been developed. It is based on the reaction between thiamine and 18-molybdodiphosphate heteropoly complex (18-MPC), which occurs at pH≈10, created by the addition of 10 % sodium carbonate. The reaction takes place at room temperature. 7-minutes are sufficient for the complete reduction of 18-MPC. The stability of heteropolyblue is limited and absorbance significantly decreases after 10 minutes of reaction. The optimal concentrations of 18-MPC and sodium carbonate were found to be 0.64 mM and 2.4 %, respectively. The ratio of 18-MPC to thiamine in the reaction is 2 : 1, which is shown by the method of continuous variations. Four electrons are accepted by the thiamine molecule during the oxidation of 18-MPC. In this case, a two-electron heteropolyblue is formed with an absorption maximum at 820 nm. The calibration dependence is linear in the range of thiamine concentrations from 2 to 80 μM with a detection limit of 0.8 μM using a cuvette with 0.5 cm path length. The proposed method has been successfully applied to the determination of vitamin B1 in three different pharmaceutical formulations containing other B vitamins, vitamin B6 and vitamin B12.
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