THE IMPACT OF ENCAPSULATED POLYPHENOLS ON THE GLYCEMIC INDEX AND ANTIOXIDANT ACTIVITY OF GLUTEN-FREE BAKED GOODS

Authors

DOI:

https://doi.org/10.15421/jchemtech.v33i3.324103

Keywords:

gluten-free bread; encapsulated polyphenols; glycemic index; antioxidant activity; functional food products.

Abstract

Gluten-free bakery products often demonstrate inferior quality parameters and may exhibit a higher glycemic index depending on the composition of the flour blend, compared to conventional products. Polyphenol extracts possess antioxidant properties and the ability to attenuate glycemic response, but they are sensitive to thermal processing. The aim of this study was to investigate the effect of incorporating encapsulated polyphenols on the glycemic index, antioxidant activity, and quality characteristics of gluten-free bread. Two groups of samples were compared: gluten-free and wheat bread (as control), each enriched with either free polyphenol extracts or the same extracts in microencapsulated form. Polyphenol losses during baking were assessed using the Folin–Ciocalteu method; antioxidant activity before and after baking was measured using the FRAP assay (expressed in Trolox equivalents). The in vitro relative glycemic index was calculated, and crumb moisture, porosity, and sensory characteristics were evaluated. The results showed that polyphenol addition enhanced antioxidant activity and contributed to a modest reduction in the glycemic index of gluten-free bread. During baking, free polyphenols underwent significant degradation (47–67 % losses in gluten-free samples), whereas encapsulation preserved a larger proportion of bioactives, reducing losses to 7–22 %. Gluten-free samples with polyphenols demonstrated a 3–5 % lower glycemic index compared to control formulations; a similar trend was observed in wheat bread. Encapsulated extracts were more effective than free forms in lowering glycemic index values. Furthermore, polyphenols improved the structural and sensory properties of the bread: specifically, they increased crumb moisture, preserved porosity in gluten-free formulations, and enhanced the color and aroma of the final products. In conclusion, encapsulated polyphenols represent promising functional ingredients for gluten-free bread, as they enhance antioxidant potential, reduce glycemic index, and improve the organoleptic quality of the product.

References

Meazza, C., Pagani, S., Gertosio, C., Bozzola, E., Bozzola, M. (2014). Celiac disease and short stature in children. Expert Review of Endocrinology & Metabolism, 9(5), 529–535. https://doi.org/10.1586/17446651.2014.932248

Rai, S., Kaur, A., Chopra, C. S. (2018). Gluten-free products for celiac susceptible people. Frontiers in Nutrition, 5, 116. https://doi.org/10.3389/fnut.2018.00116

Vici, G., Belli, L., Biondi, M., Polzonetti, V. (2016). Gluten free diet and nutrient deficiencies: A review. Clinical Nutrition, 35(6), 1236–1241. https://doi.org/10.1016/j.clnu.2016.05.002

Elli, L., Branchi, F., Tomba, C., Villalta, D., Norsa, L., Ferretti, F., Roncoroni, L., Bardella, M. T. (2015). Diagnosis of gluten related disorders: Celiac disease, wheat allergy and non-celiac gluten sensitivity. World Journal of Gastroenterology, 21(23), 7110–7119. https://doi.org/10.3748/wjg.v21.i23.7110

Gluten-Free Products Market Report. (2021). Comprehensive study explores huge growth in near future. SlideServe. https://www.slideserve.com/kailaswaghmare/gluten-free-products-market-comprehensive-study-explores-huge-growth-in-near-future

Cory, H., Passarelli, S., Szeto, J., Tamez, M., Mattei, J. (2018). The role of polyphenols in human health and food systems: A mini-review. Frontiers in Nutrition, 5, 87. https://doi.org/10.3389/fnut.2018.00087

Anton, A. A., Arntfield, S. D. (2008). Hydrocolloids in gluten-free breads: A review. International Journal of Food Sciences and Nutrition, 59(1), 11–23. https://doi.org/10.1080/09637480701625630

Scalbert, A., Johnson, I. T., Saltmarsh, M. (2005). Polyphenols: Antioxidants and beyond. The American Journal of Clinical Nutrition, 81(1 Suppl), 215S–217S. https://doi.org/10.1093/ajcn/81.1.215S

Paudel, D., Dhungana, B., Caffe, M., Krishnan, P. (2021). A review of health-beneficial properties of oats. Foods, 10(11), 2591. https://doi.org/10.3390/foods10112591

Mattioli, R., Francioso, A., Mosca, L., Silva, P. (2020). Anthocyanins: A comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules, 25(17), 3809. https://doi.org/10.3390/molecules25173809

Pravst, I., Rodríguez Aguilera, J. C., Cortes Rodriguez, A. B., Jazbar, J., Locatelli, I., Hristov, H., Žmitek, K. (2020). Comparative bioavailability of different coenzyme Q10 formulations in healthy elderly individuals. Nutrients, 12(3), 784. https://doi.org/10.3390/nu12030784

Dewettinck, K., Van Bockstaele, F., Kühne, B., Van de Walle, D., Courtens, T. M., Gellynck, X. (2008). Nutritional value of bread: Influence of processing, food interaction and consumer perception. Journal of Cereal Science, 48(2), 243–257. https://doi.org/10.1016/j.jcs.2008.01.003

Espín, J. C., García-Conesa, M. T., Tomás-Barberán, F. A. (2007). Nutraceuticals: Facts and fiction. Phytochemistry, 68(22–24), 2986–3008. https://doi.org/10.1016/j.phytochem.2007.09.014

Comettant-Rabanal, R., Piler de Carvalho, C. W., Ramirez Ascheri, J. L., Hidalgo Chávez, D. W., Gottschalk, L. M. F., & Pessanha de Araujo Santiago, M. C. (2022). Bioactive compounds of gluten-free whole and wheat breads [Resumo expandido]. II CBCP – Congresso Brasileiro de Tecnologia de Cereais e Panificação. Anais do Congresso Brasileiro de Tecnologia de Cereais e Panificação. Even3. https://doi.org/10.29327/cbcp2022.517790

Horstmann, S. W., Belz, M. C. E., Heitmann, M., Zannini, E., Arendt, E. K. (2016). Fundamental study on the impact of gluten-free starches on the quality of gluten-free model breads. Foods, 5(2), 30. https://doi.org/10.3390/foods5020030

Muscolo, A., Mariateresa, O., Giulio, T., Russo, M. (2024). Oxidative stress: The role of antioxidant phytochemicals in the prevention and treatment of diseases. International Journal of Molecular Sciences, 25(6), 3264. https://doi.org/10.3390/ijms25063264

Mitra, S., Tareq, A. M., Das, R., Emran, T. B., Nainu, F., Chakraborty, A. J. (2022). Polyphenols: A first evidence in the synergism and bioactivities. Pharmaceutical Biology, 60(1), 4419–4441. https://doi.org/10.1080/87559129.2022.2026376

Ou, J., Wang, M., Zheng, J., Ou, S. (2019). Positive and negative effects of polyphenol incorporation in baked foods. Food Chemistry, 284, 90–99. https://doi.org/10.1016/j.foodchem.2019.01.096

Ou, J. (2021). Incorporation of polyphenols in baked products. In F. Shahidi (Ed.), Advances in Food and Nutrition Research, 96, 149–183). https://doi.org/10.1016/bs.afnr.2021.02.009

Wang, Y., Maina, N. H., Coda, R., Katina, K. (2021). Challenges and opportunities for wheat alternative grains in breadmaking: Ex-situ- versus in-situ-produced dextran. Trends in Food Science & Technology, 113, 252–261. https://doi.org/10.1016/j.tifs.2021.05.003

[21] Zhang, L., McClements, D. J., Wei, Z., Wang, G., Liu, X., Liu, F. (2020). Delivery of synergistic polyphenol combinations using biopolymer-based systems: Advances in physicochemical properties, stability and bioavailability. Critical Reviews in Food Science and Nutrition, 60(12), 2083–2097. https://doi.org/10.1080/10408398.2019.1630358

Noman, A. M., Sultan, M. T., Maaz, M., Mazhar, A., Tariq, N., Imran, M., Hussain, M., Mujtaba, A., Abdelgawad, M. A., Mostafa, E. M., Ghoneim, M. M., Selim, S., Al Jbawi, E. (2025). Nutraceutical potential of anthocyanins: A comprehensive treatise. Food Science & Nutrition, 13(5), e70164. https://doi.org/10.1002/fsn3.70164

Ronda, F., Gómez, M., Blanco, C. A., Caballero, P. A. (2015). Rheological properties of gluten-free doughs: Relationship with bread quality. Food and Bioprocess Technology, 8(4), 920–930. https://doi.org/10.1016/B978-0-08-100431-9.00012-7

Pasichnyi, V. M., Hladkyi, V. M., Kolot, O. O., Stadnyk, I. V.. Tekhnolohiia khliba [Bread technology] (V. M. Pasichnyi, Ed.). Kharkiv: State Biotechnological University. (in Ukrainian) https://repo.btu.kharkov.ua/bitstream/123456789/33978/1/MV_Tekhnolohiya%20khliba_21.pdf

Sabanis, D., Tzia, C. (2011). Effect of hydrocolloids on selected properties of gluten-free dough and bread. Food and Bioproducts Processing, 89(4), 217–226. https://doi.org/10.1177/1082013210382350

Czajkowska-González, E. (2021). Addition of phenolic compounds to bread: antioxidant benefits and impact on food structure and sensory characteristics. Food Production, Processing and Nutrition, 3(25). https://doi.org/10.1186/s43014-021-00068-8

Lazaridou, A., Biliaderis, C. G. (2009). Gluten-free doughs: Rheological properties, testing procedures – methods and potential problems. In E. Gallagher (Ed.), Gluten-Free Food Science and Technology. Wiley-Blackwell. https://doi.org/10.1002/9781444316209.ch5

Mancebo, C. M., San Miguel, M. Á., Martínez, M. M., Gómez, M. (2015). Optimisation of rheological properties of gluten-free doughs with HPMC, psyllium and different levels of water. Journal of Cereal Science, 61, 8–15. https://doi.org/10.1016/j.jcs.2014.10.005

International Organization for Standardization (ISO). (2005). Determination of substances characteristic of green and black tea – Part 1: Content of total polyphenols in tea – Colorimetric method using Folin–Ciocalteu reagent. ISO 14502-1:2005. https://www.iso.org/standard/31356.html

Lee, J., Durst, R., Wrolstad, R.E. Total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines. In: Horwitz W, editor. Official Methods of Analysis of AOAC International. AOAC International.

Brand-Williams, W., Cuvelier, M. E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5

Benzie, I. F. F., Strain, J. J. (1996). The Ferric Reducing Ability of Plasma (FRAP) as a measure of "Antioxidant Power": The FRAP assay. Analytical Biochemistry, 239(1), 70–76. https://doi.org/10.1006/abio.1996.0292

Mancebo, C. M., San Miguel, M. Á., Martínez, M. M., Gómez, M. (2015). Optimisation of rheological properties of gluten-free doughs with HPMC, psyllium and different levels of water. Journal of Cereal Science, 61, 8–15. https://doi.org/10.1016/j.jcs.2014.10.005

Murariu, O. C., Caruso, G., Frunză, G., Lipșa, F. D., Ulea, E., Tallarita, A. V., Calistru, A., Jităreanu, G. (2025). Effect of wheat flour integration with blueberry fruits on rheological, quality, antioxidant, and sensory attributes of ‘French’ bread. Foods, 14(7), 1189. https://doi.org/10.3390/foods14071189

Kan, L., Oliviero, T., Verkerk, R., Fogliano, V. (2020). Interaction of bread and berry polyphenols affects starch digestibility and polyphenols bio-accessibility. Journal of Functional Foods, 68, 103924. https://doi.org/10.1016/j.jff.2020.103924

Bińkowska, W., Szpicer, A., Stelmasiak, A., Wojtasik-Kalinowska, I., Półtorak, A. (2024). Utilization of microencapsulated polyphenols to enhance the bioactive compound content in whole grain bread: Recipe optimization. Applied Sciences, 14(22), 10156. https://doi.org/10.3390/app142210156

Conte, P., Pulina, S., Del Caro, A., Fadda, C., Urgeghe, P. P., De Bruno, A., Difonzo, G., Caponio, F., Romeo, R., Piga, A. (2021). Gluten-free breadsticks fortified with phenolic-rich extracts from olive leaves and olive mill wastewater. Foods, 10(5), 1014. https://doi.org/10.3390/foods10050923

Patras, A., Brunton, N. P., O'Donnell, C., Tiwari, B. K. (2010). Effect of thermal processing on anthocyanin stability in foods: mechanisms and kinetics of degradation. Trends in Food Science & Technology, 21(1), 3–11. https://doi.org/10.1016/j.tifs.2009.07.004

Sadilova, E., Stintzing, F. C., Carle, R. (2006). Thermal degradation of anthocyanins and its impact on color and in vitro antioxidant capacity. Molecular Nutrition & Food Research, 50(4–5), 406–412. https://doi.org/10.1002/mnfr.200700179

Ronda, F., Pérez-Quirce, S., & Villanueva, M. (2016). Rheological properties of gluten-free bread doughs: Relationship with bread quality. In E. Gallagher (Ed.), Gluten-free food science and technology . Woodhead Publishing. https://doi.org/10.1016/B978-0-08-100431-9.00012-7

Sabanis, D., Tzia, C. (2011). Effect of hydrocolloids on selected properties of gluten-free dough and bread. Food Science and Technology International, 17(4), 279–291. https://doi.org/10.1177/1082013210382350

International Organization for Standardization (ISO). (2023). Sensory analysis — Selection and training of sensory assessors. ISO 8586:2023. https://www.iso.org/standard/76667.html

Published

2025-10-19