SPECTRAL CHARACTERISTICS OF FLAVONOIDS OF ROBINIA PSEUDOACACIA L. AND ROBINIA HISPIDA L. FLOWERS
DOI:
https://doi.org/10.15421/jchemtech.v33i3.326311Keywords:
flavonoids, anthocyanins, flowers of black locust and bristly locust, aluminum oxide, chemisorption, reflectance spectra, colorimetryAbstract
The results of solid-phase analysis of flowers of common black locust (Robinia pseudoacacia L.) and bristly locust (Robinia hispida L.), which are used as dietary supplements for the creation of innovative food products and medicinal plant raw materials, are presented. It has been established that a combination of characteristics of reflectance spectra, the first derivative of spectral curves, and colorimetry, which determine the distinctive features of the flowers of these species, can be used for identification. A characteristic feature of the spectral characteristics of black locust flowers is the localization of UV-absorbing flavonoids in the surface tissues. The specificity of the color of the flowers of the bristly locust tree creates an accumulation of anthocyanin pigments, for which the dominance of the flavylic form in superposition with the copigmented form has been established, which leads to the perception of a purple color. Differentiation of the spectral reflectance curve of flowers proved to be an effective way to confirm this phenomen. The spectral approaches used expand the methodological basis of non-destructive analysis of raw materials of robinia flowers for the creation of innovative food products and the improvement of pharmacognostic studies. The ability to chemisorption of flavonoids from R. pseudoacacia flower extract on aluminum oxide was established, and a biohybrid material was obtained using a biocompatible sorbent. The obtained reflectance and colorimetric characteristics of the sorption interaction product confirmed the presence of adsorbed flavonoids. The proposed solid-phase analysis of a sorbent functionalized with biologically active substances can be used to control flavonoid compositions with a solid dispersion medium.
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