EXPERIMENTAL SUBSTANTIATION OF THE METHOD OF SANITATION OF THE WATER SUPPLY SYSTEM
DOI:
https://doi.org/10.15421/jchemtech.v33i3.329631Keywords:
disinfectant; water supply system; sanitation; water safetyAbstract
Objective. The conducted research is aimed at solving the scientific problem of developing a method of sanitation of a closed water supply network based on the use of an experimental environmentally safe detergent «Sandez». The disinfectant is an aqueous alkaline solution, does not contain anti-corrosion components and compounds that pose a potential threat of negative environmental impact. The article presents the results of a comprehensive study of the antimicrobial activity of the experimental alkaline-detergent disinfectant Sandez. In vitro experiments have established bactericidal concentrations of the test disinfectant in relation to test strains of S. aureus ATCC 25923 and E. coli ATCC 25922 and S. aureus, E. coli and S. Enteritidis, previously isolated from the closed water system of poultry farms. During the experiment, the closed water supply system of the poultry house was filled with a 2.0 % solution of Sandez. In the control, a solution of sodium hypochlorite with an active chlorine concentration of 100 mg/dm³ was used to disinfect the closed water system. The decontamination solutions were left in closed systems for 5 hours, after which the solutions were drained and the lines were washed with clean tap water. It was found that the use of a 2.0 % solution of environmentally safe alkaline detergent «Sandez» for exposures of 5 hours is an effective method of sanitation of the closed water supply system of the poultry farm. The use of the disinfectant «Sandez» provides a 24 % reduction in the level of microbial contamination compared to the control. The results of sanitary and hygienic assessment of water quality in relation to oranoleptic microbiological, parasitological, physico-chemical and sanitary-toxicological indicators confirm its suitability for use in poultry farming. The introduction of this method into the system of veterinary and sanitary measures during poultry cultivation allows achieving a high level of sanitization.
References
Kukhtyn, M., Kozhyn, V., Horiuk, V., Malimon, Z., Horiuk, Y., Yashchuk, T., Kernychnyi, S. (2021). Activity of Disinfecting Biocides and Enzymes of Proteases and Amylases on Bacteria in Biofilms. Kafkas Universitesi Veteriner Fakultesi Dergisi, 27(4), 495–502. https://doi.org/10.9775/kvfd.2021.25770
Kozhyn, V., Salata, V., Kukhtyn, M., Vasylkiv, O., Laiter-Moskaliuk, S. (2024). Evaluation of the disinfectant “Enzidez” according to indicators of acute toxicity and irritant effect. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 26(113), 62–67. https://doi.org/10.32718/nvlvet11310
Kukhtyn, M., Sverhun, Z., Horiuk, Y., Salata, V., Laiter-Moskaliuk, S., Mocherniuk, M., Kladnytska, L., Horiuk, V. (2024). The influence of different methods of decontamination of microbial biofilms formed on eggshells. Slovak Journal of Food Sciences, 18, 666–682. https://doi.org/10.5219/1981
Kozhyn, M. D., Horiuk, Y. V., Horiuk, V. V., Perkiy, Y. B., Gufrij, D. F. (2021). Study of the disinfectant’s «Enzides» toxic effects on infusoria cells. Theoretical and Applied Veterinary Medicine, 9(4), 191–194. https://doi.org/10.32819/2021.94029
Kukhtyn, M., Kozhyn, V., Horiuk, V., Horiuk, Y., Boltyk, N. (2022). Evaluation of disinfectant “Enzidez” according to physical and chemical parameters. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 24(105), 3–9. https://doi.org/10.32718/nvlvet10501
Kukhtyn, M., Kozhyn, V., Horiuk, V., Malimon, Z., Horiuk, Y., Yashchuk, T., Kernychnyi, S. (2021). Activity of Disinfecting Biocides and Enzymes of Proteases and Amylases on Bacteria in Biofilms. Kafkas Universitesi Veteriner Fakultesi Dergisi, 27(4), 495–502. https://doi.org/kvfd.2021.25770
Kasіanenko, O. I., Nesterenko, О. М. (2023). Study of the efficiency of using the experimental biocide sandez for the sanitation of the poultry water supply system. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 24(2), 72–76. https://doi.org/10.36359/scivp.2023-24-2.08
Dolbanosova, R. V., Nahorna, L. V., Serhiienko, Ya. V. (2023). Comparative characteristics of some indicators works of the hypochlorite installation in the process of water disinfection. Bulletin of Sumy National Agrarian University. The series: Veterinary Medicine, 4(63). 30–36. https://doi.org/10.32782/bsnau.vet.2023.4.5
Ghozhenko, A. I., Petrenko, N. F., Mokijenko, A. V. (2008). [Biological foundations of economic safety of using chemical means for disinfection of drinking water] (Literature review and own research)]. Zhurnal Akademiji medychnykh nauk, 14(1), 134–149. (In Ukrainian)
Kramarenko, L. V. (2010). [Special course on natural water treatment]. Kharkiv: KNAUE. (In Ukrainian)
Petruk, V. Gh., Styskal, O. A. (2016). [Analysis of current safe alternatives for drinking ater disinfection]. Ekologhichni nauky, 1–2(12–13), 50–57. (In Ukrainian).
Research Center «Polymer Pipelines in Construction» (2012). [Guidelines for the construction, installation and quality control of pipelines of external water supply and sewage networks]. (DSTU-N B V.2.5-68:2012). Kyiv, Derzhstandart Ukraine (in Ukrainian).
Instrukcija z dezinfekcij vodoprovidnykh merezh ta sporud na nykh. (In Ukrainian) https://zakon.rada.gov.ua/laws/show/z0145-00#Text
Ponomar, S. I., Artemenko, L. P, Lytvynenko, O. P., Ghoncharenko, V. P. (2011). [Handbook on laboratory methods for diagnosing invasive animal diseases]. Bila Cerkva. (In Ukrainian).
Mafijevsjkyj, V. F., Serdjuk, A. M. (2006). [New water treatment technologies from the perspective of the World Health Organization's concept of «Risk Management»]. Voda i vodoochysni tekhnologhiji, 3: 23–29. (In Ukrainian).
Kovalenko, V. L. (2019). [Guidelines for the determination of bactericidal activity and control of the absence of bacteriostatic effect of disinfectants], Metodychni rekomendaciji, 28. (In Ukrainian).
Abdelaty, M.F., Nasr, S.A.E. Hamoud, M. M., Ismail, T. F., Laban, S. E., Gamal, A., Bashandy, E. Y., Nasefand, S. A., Zahran, O. K. (2019). Efficiency of some sanitizers and disinfectants against biofilms and planktonic cells buildup oncages (Galvanized wire) and plastic material (PVC) in poultry farms. Inter J., Vet Sci., 8(3), 120–126.
Kasіanenko, O. I., Naghorna, L. V., Kasіanenko, S. M. (2020). Efficiency of the use of washing and disinfectant «Sandez» for disinfection of poultry houses. Bulletin of Sumy National Agrarian University. The series: Veterinary Medicine, 2 (49). 16–23.
Kasjanenko, O.I. Naghorna, L.V. Kasjanenko, S.M. Nesterenko O.M. (2006). Pat. 154333 Ukrajiny, MPK (2006): C11D 7/00, A61L 9/00, A61L 2/00. [Method of renovating the poultry farm water supply system] (In Ukrainian)
Kovalenko, V. L., Harkavenko, T. O., Horbatiuk, O. I., Kozytska, T. H., Harkavenko, V. M., Ordynska, D. O. (2019). [Guidelines for the determination of bactericidal activity and control of the absence of bacteriostatic effect of disinfectants]. Kyiv. (In Ukrainian)
Instructions for sanitary treatment - disinfection, disinsection and deratization of poultry facilities https://zakon.rada.gov.ua/laws/show/z0813-07#Text
Instructions for disinfection of water supply networks and structures on them https://zakon.rada.gov.ua/laws/show/z0145-00#Text
State sanitary norms and rules «Hygienic requirements for drinking water intended for human consumption» https://zakon.rada.gov.ua/laws/show/z0452-10#Text
Ukrainian Research Institute of Environmental Problems. (2010). [Water quality. Sampling]. (DSTU ISO 5667-2009). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).
Technical Committee for Standardization "Alcoholic Beverages, Yeast" (2004). [Water quality. Determination and investigation of turbidity]. (DSTU ISO 7027–2003). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).
Technical Committee for Standardization "Alcoholic Beverages, Yeast" (2005). [Water quality. Determination and investigation of coloration]. (DSTU 7887–2003). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).
Ukrainian Research Institute of Environmental Problems. (2003). [Water quality. Determination of pH]. (ДСТУ ISO 4077). Kyiv, Derzhstandart Ukrainе (In Ukrainian).
Institute of Water Problems and Land Reclamation of the National Academy of Agrarian Sciences (2004). [Water quality. Determination of ammonium. Potentiometric method]. (DSTU ISO 6778:2003 (ISO 6778-1984, IDT). Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).
Institute of Water Problems and Land Reclamation of the National Academy of Agrarian Sciences (2009). [Water quality. Determination of total hardness. Part 2]. (DSTU ISO 9963–2:2007), Kyiv, Derzhpozhyvstandart Ukraine (in Ukrainian).
Ukrainian Research and Training Center for Standardization, Certification and Quality Problems (2007). [Water quality. Identification and accounting of Esherichia coli and coliform bacteria. PART 1. Membrane filtration method]. (DSTU ISO 9308–1: 2005). Kyiv, Derzhstandart Ukraine (in Ukrainian).
Ukrainian Research Institute of Environmental Problems (2008). [Water quality. Identification and accounting for coliform bacteria and estimated amounts of Esherichia сoli. - PART 2. Multiple tube method]. (DSTU ISO 9308–2: 2005 Kyiv, Derzhstandart Ukraine (in Ukrainian).
Methodological guidelines «Sanitary and microbiological control of drinking water quality» https://zakon.rada.gov.ua/rada/show/v0060282-05#Text
State Sanitary Norms and Rules «Hygienic Requirements for Drinking Water Intended for Human Consumption»
https://zakon.rada.gov.ua/laws/show/z0452-10#Text
European Convention for the protection of vertebrate animals used for research and other scientific purposes https://zakon.rada.gov.ua/laws/show/994_137#Text
Procedure for conducting experiments on animals by scientific institutions https://zakon.rada.gov.ua/laws/show/z0416-12#Text
European convention for the protection of vertebrate animals used for experimental and other scientific purposes. (1986, March). https://rm.coe.int/168007a67b
Kasianenko, O. I., Nesterenko, O. M. (2024). Alternative methods of prevention of infectious diseases of poultry. Bulletin of Sumy National Agrarian University. The series: Veterinary Medicine, 2(65), 13–22. https://doi.org/10.32782/bsnau.vet.2024.2.3
Kasianenko, O. I., Shvets, Kh. S., Dolbanosova, R. V. (2024). Alternative methods of prevention of infectious poultry diseases. Bulletin of Sumy National Agrarian University. The series: Veterinary Medicine, 1(64), 3–10. https://doi.org/10.32782/bsnau.vet.2024.1.1
Liu, Zh., Yu, Y., Fotina, T., Petrov, R., Klishchova, Zh., Fotin, A., Ma J. (2022). Multiplex PCR assay based on the citE2 gene and intergenic sequence for the rapid detection of Salmonella Pullorum in chickens. Poultry Science, 101(8), 101981
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