QUALIFICATION SYSTEM FOR COMPREHENSIVE IDENTIFICATION OF HOP OIL
DOI:
https://doi.org/10.15421/jchemtech.v34i1.349864Keywords:
ethanol extract; essential oil; hops; spectrophotometry; authentication; terpenes; quality.Abstract
Modern technological capabilities are causing concern due to the creativity of approaches to the falsification of food products and their components, in particular essential oils. The struggle for quality and safety of end products calls on the global scientific community to improve and create more diverse methodologies with mandatory mathematical comparative analysis of the impact on the same system under changing influencing factors. The aim of the research was to create additional criteria for preventing falsification through spectrophotometric analysis of the intensity of light absorption by ethanol solutions of essential oils of bitter hops. Individual graphical-positional anomalies of wavelengths and spectral intervals were determined, and equations of functions of changes in the concentration of terpene complexes in the studied samples were calculated, coefficients were determined, and the error values were experimentally proven. A rational ratio (oil-ethanol) for dissolution without opalescence was empirically established. The spectra and changes in the optical density of hop oil were evaluated in basic units of light absorption and in percentages in the wavelength range from 190 nm to 900 nm discretely, with a wavelength determination step of 0.05–1 nm. Based on the study of known-quality samples, a wavelength range of 456–780 nm was established, at which the fluctuation in the intensity of the absorbing activity of the main terpenoid compounds facilitates the authentication of other samples of ethanol solutions of hop oils. In addition, fluctuations in the coefficients of mathematical systems for monitoring changes in light absorption intensity in ethanol solutions of hop oil provide an opportunity for comparative control of these changes and, accordingly, create additional criteria for preventing counterfeiting.
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