DETERMINATION OF ASCORBIC ACID IN MIXTURES WITH CYSTEINE USING REDUCTION OF 18-MOLYBDODIPHOSPHATE HETEROPOLY COMPLEX IN PRESENCE OF Bi(III) IONS

Authors

  • Yuliia V. Miekh Дніпровський національний університет імені Олеся Гончара, Ukraine
  • Andriy B. Vishnikin Oles Honchar Dnipro National University, Ukraine
  • Nataliya A. Tovstonog Дніпровський національний університет імені Олеся Гончара,
  • Tatyana A. Denisenko Дніпровський національний університет імені Олеся Гончара,
  • Halyna O. Petrushyna Дніпровський державний аграрно-економічний університет,

DOI:

https://doi.org/10.15421/081929

Keywords:

ascorbic acid, cysteine, spectrophotometry, 18-molybdodiphosphate, simultaneous determination.

Abstract

A spectrophotometric method was developed for the determination of ascorbic acid in mixtures with cysteine using the reduction of 18-molybdodiphosphate heteropoly complex. In order to decrease the influence of the 18-molybdodiphosphate reduction with cysteine, the pH of the reaction was shifted to the acidic region to 1.0 – 2.2. Under these conditions, in the presence of bismuth(III) ions, as a result of the formation of a metal-substituted heteropoly complex, the reaction rate of 18-molybdodiphosphate with ascorbic acid increases and, at pH from 1.8 to 2.2, the reduction reaction is completely shifted towards the formation of heteropoly blue and ends in less than 5 minutes. Cysteine in this pH region reacts with 18-molybdodiphosphate very slowly, but has an indirect effect on the reaction of ascorbic acid with 18-molybdodiphosphate, probably due to the binding of bismuth(III) ions. It was shown that the determination of ascorbic acid is possible with satisfactory accuracy if the excess of cysteine with respect to ascorbic acid does not exceed three times. The calibration graph for the determination of ascorbic acid at pH 1.8 and concentrations of 18-molybdodiphosphate and bismuth(III) 5∙10-5 mol∙L-1 and 1∙10-4 mol∙L-1, respectively, is linear in the range from 2.5∙10-6 to 2.5∙10-5 with a detection limit of 8∙10-7 mol∙L-1 (l = 5 cm). The accuracy of the method was evaluated by the added-found method on model mixtures.

Author Biographies

Yuliia V. Miekh, Дніпровський національний університет імені Олеся Гончара

Молодший науковий співробітник Інституту хімії та геології ДНУ

Andriy B. Vishnikin, Oles Honchar Dnipro National University

Head of Analytical Chemistry Department h-index (SCOPUS) = 8

Nataliya A. Tovstonog, Дніпровський національний університет імені Олеся Гончара

Магістр 6 курс

Tatyana A. Denisenko, Дніпровський національний університет імені Олеся Гончара

Доцент кафедри фізичної, неорганічної та органічної хімії ДНУ

Halyna O. Petrushyna, Дніпровський державний аграрно-економічний університет

Доцент

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Published

2020-01-21

Issue

Vol. 27 No. 2 (2019): Journal of Chemistry and Technologies

Section

Analytical Chemistry

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